Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-25452
Recombinant Monoclonal Antibody
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Key Product Details
Species Reactivity
Virus
Applications
Western Blot, ELISA, Sandwich ELISA, Lateral Flow Assay
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1997 expressed in HEK293
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant fragment of Enterovirus D68 VP1
Reactivity Notes
Enterovirus D68
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free
ELISA: Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free [NBP3-25452] -
ELISA: Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free [NBP3-25452] - Sandwich ELISA detection of recombinant full-length VP1 protein(s) derived from different strains of Enterovirus (ie., D68; 71) using antibodies as below. Capture: Enterovirus D68 VP1 antibody [GT11610] (NBP3-13557) (5 ug/mL) Detection: Enterovirus D68 VP1 antibody [HL1997] (NBP3-25452) (1 ug/mL)Lateral Flow Assay: Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free [NBP3-25452] -
Lateral Flow Assay: Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free [NBP3-25452] - Detection of enterovirus D68 VP1 protein by lateral flow assay using the monoclonal antibody pair. Capture: Enterovirus D68 VP1 antibody (NBP3-25452 [HL1997]) Detection: Enterovirus D68 VP1 antibody (NBP3-13564 [GT1843]) Samples (80 ng) : 1. Enterovirus D68 VP1 protein 2. Lysis bufferWestern Blot: Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free [NBP3-25452] -
Western Blot: Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free [NBP3-25452] - Non-infected (-) and infected (+) RD whole cell extracts (20 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus D68 VP1 antibody [HL1997] (NBP3-25452) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Western Blot: Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free [NBP3-25452] -
Western Blot: Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free [NBP3-25452] - Non-infected (-) and infected (+) RD whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus D68 VP1 antibody [HL1997] (NBP3-25452) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.ELISA: Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free [NBP3-25452] -
ELISA: Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free [NBP3-25452] - Sandwich ELISA detection of recombinant full-length VP1 protein(s) derived from different strains of Enterovirus (ie., D68; 71) using antibodies as below. Capture: Enterovirus D68 VP1 antibody [GT1843] (NBP3-13564) (5 ug/mL) Detection: Enterovirus D68 VP1 antibody [HL1997] (NBP3-25452) (1 ug/mL)Lateral Flow Assay: Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free [NBP3-25452] -
Lateral Flow Assay: Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free [NBP3-25452] - Detection of enterovirus D68 VP1 protein by lateral flow assay using the monoclonal antibody pair. Capture: Enterovirus D68 VP1 antibody (NBP3-13564 [GT1843]) Detection: Enterovirus D68 VP1 antibody (NBP3-25452 [HL1997]) Samples (80 ng) : 1. Enterovirus D68 VP1 protein 2. Lysis bufferImmunocytochemistry/ Immunofluorescence: Enterovirus D68 VP1 Antibody (HL1997) [NBP3-25452] -
Enterovirus D68 VP1 antibody [HL1997] detects Enterovirus D68 VP1 protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Enterovirus D68 VP1 stained by Enterovirus D68 VP1 antibody [HL1997] (NBP3-25452) diluted at 1:2000.
Blue: Fluoroshield with DAPI.
Western Blot: Enterovirus D68 VP1 Antibody (HL1997) [NBP3-25452] -
Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus D68 VP1 antibody [HL1997] (NBP3-25452) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Enterovirus D68 VP1
Alternate Names
Capsid protein VP1, EV68 VP1, P1D, Virion protein 1
Additional Enterovirus D68 VP1 Products
Product Documents for Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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