Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25452

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Virus

Applications

Western Blot, ELISA, Sandwich ELISA, Lateral Flow Assay

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1997 expressed in HEK293

Format

Azide and BSA Free
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Product Specifications

Immunogen

Recombinant fragment of Enterovirus D68 VP1

Reactivity Notes

Enterovirus D68

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free

Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free

ELISA: Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free [NBP3-25452] -

ELISA: Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free [NBP3-25452] - Sandwich ELISA detection of recombinant full-length VP1 protein(s) derived from different strains of Enterovirus (ie., D68; 71) using antibodies as below. Capture: Enterovirus D68 VP1 antibody [GT11610] (NBP3-13557) (5 ug/mL) Detection: Enterovirus D68 VP1 antibody [HL1997] (NBP3-25452) (1 ug/mL)
Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free

Lateral Flow Assay: Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free [NBP3-25452] -

Lateral Flow Assay: Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free [NBP3-25452] - Detection of enterovirus D68 VP1 protein by lateral flow assay using the monoclonal antibody pair. Capture: Enterovirus D68 VP1 antibody (NBP3-25452 [HL1997]) Detection: Enterovirus D68 VP1 antibody (NBP3-13564 [GT1843]) Samples (80 ng) : 1. Enterovirus D68 VP1 protein 2. Lysis buffer
Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free

Western Blot: Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free [NBP3-25452] -

Western Blot: Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free [NBP3-25452] - Non-infected (-) and infected (+) RD whole cell extracts (20 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus D68 VP1 antibody [HL1997] (NBP3-25452) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free

Western Blot: Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free [NBP3-25452] -

Western Blot: Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free [NBP3-25452] - Non-infected (-) and infected (+) RD whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus D68 VP1 antibody [HL1997] (NBP3-25452) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free

ELISA: Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free [NBP3-25452] -

ELISA: Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free [NBP3-25452] - Sandwich ELISA detection of recombinant full-length VP1 protein(s) derived from different strains of Enterovirus (ie., D68; 71) using antibodies as below. Capture: Enterovirus D68 VP1 antibody [GT1843] (NBP3-13564) (5 ug/mL) Detection: Enterovirus D68 VP1 antibody [HL1997] (NBP3-25452) (1 ug/mL)
Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free

Lateral Flow Assay: Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free [NBP3-25452] -

Lateral Flow Assay: Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free [NBP3-25452] - Detection of enterovirus D68 VP1 protein by lateral flow assay using the monoclonal antibody pair. Capture: Enterovirus D68 VP1 antibody (NBP3-13564 [GT1843]) Detection: Enterovirus D68 VP1 antibody (NBP3-25452 [HL1997]) Samples (80 ng) : 1. Enterovirus D68 VP1 protein 2. Lysis buffer
Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Enterovirus D68 VP1 Antibody (HL1997) [NBP3-25452] -

Enterovirus D68 VP1 antibody [HL1997] detects Enterovirus D68 VP1 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Enterovirus D68 VP1 stained by Enterovirus D68 VP1 antibody [HL1997] (NBP3-25452) diluted at 1:2000.
Blue: Fluoroshield with DAPI.
Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free

Western Blot: Enterovirus D68 VP1 Antibody (HL1997) [NBP3-25452] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus D68 VP1 antibody [HL1997] (NBP3-25452) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Enterovirus D68 VP1

Alternate Names

Capsid protein VP1, EV68 VP1, P1D, Virion protein 1

Additional Enterovirus D68 VP1 Products

Product Documents for Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Enterovirus D68 VP1 Antibody (HL1997) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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