EphA3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-16346

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (97%), Chicken (92%), Porcine (97%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all EphA3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human EphA3. The exact sequence is proprietary.

Localization

Isoform 1: Cell membrane; Single-pass type I membrane protein; Isoform 2: Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EphA3 Antibody - BSA Free

Western Blot: EphA3 Antibody [NBP2-16346]

Western Blot: EphA3 Antibody [NBP2-16346]

Western Blot: EphA3 Antibody [NBP2-16346] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA3 antibody [N1N3] diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Immunohistochemistry-Paraffin: EphA3 Antibody [NBP2-16346]

Immunohistochemistry-Paraffin: EphA3 Antibody [NBP2-16346]

Immunohistochemistry-Paraffin: EphA3 Antibody [NBP2-16346] - Human gastric cancer, using Eph Receptor A3 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Western Blot: EphA3 Antibody [NBP2-16346]

Western Blot: EphA3 Antibody [NBP2-16346]

Western Blot: EphA3 Antibody [NBP2-16346] - Mouse tissue extract (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA3 antibody [N1N3] diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: EphA3 Antibody [NBP2-16346]

Western Blot: EphA3 Antibody [NBP2-16346]

Western Blot: EphA3 Antibody [NBP2-16346] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA3 antibody [N1N3] diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
EphA3 Antibody

Western Blot: EphA3 Antibody [NBP2-16346] -

Western Blot: EphA3 Antibody [NBP2-16346] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA3 antibody [N1N3] (NBP2-16346) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
EphA3 Antibody - BSA Free

Western Blot: EphA3 Antibody [NBP2-16346] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA3 antibody [N1N3] (NBP2-16346) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Applications for EphA3 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS

Format

BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: EphA3

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Two alternatively spliced transcript variants have been described for this gene. [provided by RefSeq]

Long Name

Eph Receptor A3

Alternate Names

Cek4, Hek4, Tyro4

Gene Symbol

EPHA3

UniProt

P29320 (Human)

Additional EphA3 Products

Product Documents for EphA3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for EphA3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for EphA3 Antibody - BSA Free

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  • Q: Can we use NBP2-16346  for IP?

    A: We have not validated EphA3 Antibody NBP2-16346 for IP so we do not know for certain if it will work. If you decide to buy and test it you can take advantage of our Innovators Rewards Program for unvalidated applications.

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