EphA4 Antibody

Novus Biologicals | Catalog # NBP2-16348

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Chicken (96%), Porcine (99%), Rhesus Macaque (100%), Xenopus (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all EphA4 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human EphA4. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (92%).

Localization

Membrane; Single-pass type I membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EphA4 Antibody

Western Blot: EphA4 Antibody [NBP2-16348]

Western Blot: EphA4 Antibody [NBP2-16348]

Western Blot: EphA4 Antibody [NBP2-16348] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA4 antibody, Internal diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: EphA4 Antibody [NBP2-16348]

Western Blot: EphA4 Antibody [NBP2-16348]

Western Blot: EphA4 Antibody [NBP2-16348] - Sample (30 ug of whole cell lysate) A: HeLa 7. 5% SDS PAGE gel, diluted at 1:1000.
Western Blot: EphA4 Antibody [NBP2-16348]

Western Blot: EphA4 Antibody [NBP2-16348]

Western Blot: EphA4 Antibody [NBP2-16348] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA4 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
EphA4 Antibody

Western Blot: EphA4 Antibody [NBP2-16348] -

Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA4 antibody [N3C2], Internal (NBP2-16348) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
EphA4 Antibody

Western Blot: EphA4 Antibody [NBP2-16348] -

Eph receptor A4 antibody [N3C2-2], Internal detects Eph receptor A4 protein by western blot analysis. Whole cell extracts (30 ug) was separated by 5 % SDS-PAGE, and blotted with Eph receptor A4 antibody [N3C2-2], Internal (NBP2-16348) diluted by 1:1000

Applications for EphA4 Antibody

Application
Recommended Usage

Immunohistochemistry

Assay dependent

Immunohistochemistry-Paraffin

Assay dependent

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: EphA4

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. [provided by RefSeq]

Long Name

Eph Receptor A4

Alternate Names

Cek8, Hek8, Sek1, Tyro1

Gene Symbol

EPHA4

UniProt

P54764 (Human)

Additional EphA4 Products

Product Documents for EphA4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for EphA4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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