Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot

Label

Janelia Fluor 549

Antibody Source

Monoclonal Rat IgG1 Clone # 512013
View all EphB2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse EphB2
Val27-Lys548
Accession # P54763

Specificity

Detects mouse EphB2 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human EphB2 is observed. In Western blots, no cross-reactivity with recombinant human EphB2 is observed. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse (rm) EphA1, 2, 3, 4, 5, 6, 7, 8, rmEphB4, 6, recombinant human EphA10, recombinant rat (rr) EphA10, or rrEphB1 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Applications for EphB2 Antibody (512013) [Janelia Fluor® 549]

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: EphB2

Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members.

Long Name

Eph Receptor B2

Alternate Names

Cek5, Drt, Erk, Hek5, Nuk, Qek2, Sek3, Tyro5

Gene Symbol

EPHB2

Additional EphB2 Products

Product Documents for EphB2 Antibody (512013) [Janelia Fluor® 549]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for EphB2 Antibody (512013) [Janelia Fluor® 549]



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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