Ephrin-A3 Antibody (OTI4A4)

Novus Biologicals | Catalog # NBP2-46484

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI4A4
View all Ephrin-A3 Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 23-214 of human EFNA3(NP_004943) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

26.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Ephrin-A3 Antibody (OTI4A4)

Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) [NBP2-46484]

Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) [NBP2-46484]

Immunohistochemistry: Ephrin-A3 Antibody (4A4) [NBP2-46484] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) [NBP2-46484]

Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) [NBP2-46484]

Immunohistochemistry: Ephrin-A3 Antibody (4A4) [NBP2-46484] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) [NBP2-46484]

Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) [NBP2-46484]

Immunohistochemistry: Ephrin-A3 Antibody (4A4) [NBP2-46484] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) [NBP2-46484]

Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) [NBP2-46484]

Immunohistochemistry: Ephrin-A3 Antibody (4A4) [NBP2-46484] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) [NBP2-46484]

Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) [NBP2-46484]

Immunohistochemistry: Ephrin-A3 Antibody (4A4) [NBP2-46484] - Analysis of Carcinoma of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) [NBP2-46484]

Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) [NBP2-46484]

Immunohistochemistry: Ephrin-A3 Antibody (4A4) [NBP2-46484] - Analysis of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) [NBP2-46484]

Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) [NBP2-46484]

Immunohistochemistry: Ephrin-A3 Antibody (4A4) [NBP2-46484] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Ephrin-A3 Antibody (OTI4A4)

Immunohistochemistry-Paraffin: Ephrin-A3 Antibody (OTI4A4) [NBP2-46484] -

Immunohistochemistry-Paraffin: Ephrin-A3 Antibody (OTI4A4) [NBP2-46484] - Ephrin-A3 immunoreactivity in mouse trigeminal ganglion neurons. Sections were incubated with the antibody overnight at 4 degrees. Biotinylated mouse IgG was used to help verify the specificity of the antibody (NBP1-97078). Biotin conjugated version of antibody used (NBP2-70623B). Image from verified customer review.

Applications for Ephrin-A3 Antibody (OTI4A4)

Application
Recommended Usage

Immunohistochemistry

1:150

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Ephrin-A3

The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis

Alternate Names

EFL-2, EFNA3, Ehk1-L, EphrinA3, LERK3

Gene Symbol

EFNA3

Additional Ephrin-A3 Products

Product Documents for Ephrin-A3 Antibody (OTI4A4)

Certificate of Analysis

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Product Specific Notices for Ephrin-A3 Antibody (OTI4A4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Ephrin Ligands and Eph Receptors

Citations for Ephrin-A3 Antibody (OTI4A4)

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Protocols

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