Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-70623

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI4A4

Format

Azide and BSA Free
View all Ephrin-A3 Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 23-214 of human EFNA3(NP_004943) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

26.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free

Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623]

Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623]

Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623] - Analysis of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623]

Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623]

Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623]

Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623]

Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623]

Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623]

Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623] - Analysis of Carcinoma of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623]

Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623]

Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623]

Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623]

Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623]

Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623]

Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free

Immunohistochemistry-Paraffin: Mouse Ephrin-A3 mAb (OTI4A4) - Azide and BSA Free [NBP2-70623] -

Immunohistochemistry-Paraffin: Mouse Ephrin-A3 mAb (OTI4A4) - Azide and BSA Free [NBP2-70623] - Ephrin-A3 immunoreactivity in mouse trigeminal ganglion neurons. Sections were incubated with the antibody overnight at 4 degrees. Biotinylated mouse IgG was used to help verify the specificity of the antibody (NBP1-97078). Biotin conjugated version of antibody used (NBP2-70623B). Image from verified customer review.

Applications for Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ephrin-A3

The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis

Alternate Names

EFL-2, EFNA3, Ehk1-L, EphrinA3, LERK3

Gene Symbol

EFNA3

Additional Ephrin-A3 Products

Product Documents for Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Ephrin Ligands and Eph Receptors

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Protocols

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FAQs

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