EPS15 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-12544

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Eps15 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human EPS15 fragment

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Protein A & Antigen Affinity purified

Scientific Data Images for EPS15 Antibody - BSA Free

Western Blot: EPS15 Antibody [NBP3-12544]

Western Blot: EPS15 Antibody [NBP3-12544]

Western Blot: EPS15 Antibody [NBP3-12544] - Lane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:99 kDa(We are unsure as to the identity of these extra bands.)
Immunohistochemistry-Paraffin: EPS15 Antibody [NBP3-12544]

Immunohistochemistry-Paraffin: EPS15 Antibody [NBP3-12544]

Immunohistochemistry-Paraffin: EPS15 Antibody [NBP3-12544] - Staining of human EPS15 in human lymph node with rabbit polyclonal antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: EPS15 Antibody [NBP3-12544]

Immunohistochemistry-Paraffin: EPS15 Antibody [NBP3-12544]

Immunohistochemistry-Paraffin: EPS15 Antibody [NBP3-12544] - Staining of human EPS15 in human stomach with rabbit polyclonal antibody at 1:500 dilution.
Immunoprecipitation: EPS15 Antibody [NBP3-12544]

Immunoprecipitation: EPS15 Antibody [NBP3-12544]

Immunoprecipitation: EPS15 Antibody [NBP3-12544] - Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-EPS15 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-EPS15 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 99 kDaObserved band size :150 kDa

Applications for EPS15 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Immunoprecipitation

1-5uL/mg of uLysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Format

BSA Free

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Eps15

EPS15 is a ubiquitously expressed protein that is involved in cell growth regulation and may be involved in the regulation of mitogenic signals and control of cell proliferation. EPS15 may be phosphorylated on tyrosine 849 following DNA damage, resulting in the internalization of EGFR. A chromosomal aberration involving EPS15 is also found in acute myelogeneous leukemias, resulting in a rogue activator protein, so EPS15 antibodies may be useful for cancer research.

Long Name

EGFR Pathway Substrate 15

Alternate Names

AF1P, MLLT5

Gene Symbol

EPS15

Additional Eps15 Products

Product Documents for EPS15 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for EPS15 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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