Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG3 Kappa Clone # 18F5B11
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Product Specifications
Immunogen
Amino acids 701-882 of human EPS8 protein were used as the immunogen for the EPS8 antibody.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG3 Kappa
Description
Novus Biologicals Mouse Eps8 Antibody (18F5B11) (NBP2-27344) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Eps8 Antibody (18F5B11)
![Western Blot: Eps8 Antibody (18F5B11) [NBP2-27344]](https://resources.rndsystems.com/images/products/EPS8-Antibody-18F5B11-Western-Blot-NBP2-27344-img0001.jpg "Western Blot: Eps8 Antibody (18F5B11) [NBP2-27344]")
Western Blot: Eps8 Antibody (18F5B11) [NBP2-27344]
Western Blot: EPS8 Antibody (18F5B11) [NBP2-27344] - analysis of EPS8 partial recombinant protein using EPS8 antibody at 1 ug/ml.![Immunohistochemistry-Paraffin: Eps8 Antibody (18F5B11) [NBP2-27344]](https://resources.rndsystems.com/images/products/EPS8-Antibody-18F5B11-Immunohistochemistry-Paraffin-NBP2-27344-img0002.jpg "Immunohistochemistry-Paraffin: Eps8 Antibody (18F5B11) [NBP2-27344]")
Immunohistochemistry-Paraffin: Eps8 Antibody (18F5B11) [NBP2-27344]
Immunohistochemistry-Paraffin: EPS8 Antibody (18F5B11) [NBP2-27344] - Formalin-fixed, paraffin-embedded human salivary gland stained with EPS8 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen.Applications for Eps8 Antibody (18F5B11)
Application
Recommended Usage
Immunohistochemistry-Paraffin
5ug/ml
Western Blot
1-5ug/ml~
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Eps8
Long Name
Epidermal Growth Factor Receptor Pathway Substrate 8
Alternate Names
epidermal growth factor receptor kinase substrate 8, epidermal growth factor receptor pathway substrate 8
Gene Symbol
EPS8
UniProt
Additional Eps8 Products
Product Documents for Eps8 Antibody (18F5B11)
Product Specific Notices for Eps8 Antibody (18F5B11)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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