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Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 665 and 715 of human epidermal growth factor receptor pathway substrate 8-like protein 2 using the numbering given in entry NP_073609.2 (GeneID 64787).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for EPS8L2 Antibody
Western Blot: EPS8L2 Antibody [NB100-97850]
Western Blot: EPS8L2 Antibody [NB100-97850] - Detection of Human EPS8L2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EPS8L2 antibody NB100-97850 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Immunoprecipitation: EPS8L2 Antibody [NB100-97850]
Immunoprecipitation: EPS8L2 Antibody [NB100-97850] - Detection of human EPS8L2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EPS8L2 antibody NB100-97850 (lot 2) used for IP at 6 ug per reaction. EPS8L2 was also immunoprecipitated by a previous lot of this antibody (lot 1) and rabbit anti-EPS8L2 antibody NB100-97849. For blotting immunoprecipitated EPS8L2, NB100-97849 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Applications for EPS8L2 Antibody
Application
Recommended Usage
Immunoprecipitation
1:10-1:500
Western Blot
1:100-1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: EPS8L2
Alternate Names
epidermal growth factor receptor kinase substrate 8-like protein 2, Epidermal growth factor receptor pathway substrate 8-related protein 2, EPS8-like 2, EPS8-like protein 2, EPS8R2, EPS8-related protein 2, FLJ16738, FLJ21935, FLJ22171, MGC126530, MGC3088
Entrez Gene IDs
64787 (Human)
Gene Symbol
EPS8L2
UniProt
Additional EPS8L2 Products
Product Documents for EPS8L2 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for EPS8L2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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