ErbB2/Her2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-02960

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ErbB2/Her2 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1156-1255 of human ErbB2/Her2 (NP_004439.2). PTVPLPSETDGYVAPLTCSPQPEYVNQPDVRPQPPSPREGPLPAARPAGATLERPKTLSPGKNGVVKDVFAFGGAVENPEYLTPQGGAAPQPHPPPAFSPAFDNLYYWDQDPPERGAPPSTFKGTPTAENPEYLGLDVPV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ErbB2/Her2 Antibody - BSA Free

Western Blot: ErbB2/Her2 AntibodyBSA Free [NBP3-02960]

Western Blot: ErbB2/Her2 AntibodyBSA Free [NBP3-02960]

Western Blot: ErbB2/Her2 Antibody [NBP3-02960] - Analysis of extracts of Mouse lung, using HER2 / ErbB2 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody - BSA Free [NBP3-02960]

Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody - BSA Free [NBP3-02960]

Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP3-02960] - Rat testis using [KO Validated] HER2 / ErbB2 Rabbit pAb at dilution of 1:100 (40x lens).
Western Blot: ErbB2/Her2 AntibodyBSA Free [NBP3-02960]

Western Blot: ErbB2/Her2 AntibodyBSA Free [NBP3-02960]

Western Blot: ErbB2/Her2 Antibody [NBP3-02960] - Analysis of extracts of SK-BR-3 cells, using HER2 / ErbB2 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 1s.

Applications for ErbB2/Her2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:100 - 1:500

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ErbB2/Her2

ErbB2/HER2 is one of the four members of the ErbB receptor family of transmembrane receptor-like tyrosine kinases (1). The kinase activity of ErbB2 can be activated without ligand if it is overexpressed, and by association with other ErbB proteins (2). Overexpression of ErbB2 is detected in almost 40% of human breast cancers (3). Binding of c-Cbl ubiquitin ligase to Tyr1112 of ErbB2 leads to poly-ubiquitination of ErbB2 and enhances its degradation (4). ErbB2 is one of the major targets for the treatment of breast cancer and other carcinomas.

Long Name

Receptor Tyrosine Protein Kinase ErbB2

Alternate Names

CD340, HER2, Neu Oncogene, NGL, TKR1

Gene Symbol

ERBB2

Additional ErbB2/Her2 Products

Product Documents for ErbB2/Her2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ErbB2/Her2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs for ErbB2/Her2 Antibody - BSA Free

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  • Q: I am interested in getting antibodies for HER that would work in flow cytometry. Could you recommend some choices?

    A:

    A list of our ErbB2 antibodies validated for use in flow cytometry can be found here /productsearch/HER2#fq=common_name%3A%22ErbB2%2FHER2%22&fq=category%3A%22Primary%20Antibodies%22&fq=applications%3A%22Flow%20Cytometry%22

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