ErbB3/Her3 [p Tyr1222] Antibody (5Z1F0)

Novus Biologicals | Catalog # NBP3-16922

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5Z1F0 expressed in HEK293
View all ErbB3/Her3 Primary Antibodies »

Product Specifications

Immunogen

A synthetic phosphorylated peptide around Y1222 of human ErbB3/Her3 (P21860). LGYEY

Modification

p Tyr 1222

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ErbB3/Her3 [p Tyr1222] Antibody (5Z1F0)

Western Blot: ErbB3/Her3 [p Tyr1222] Antibody (5Z1F0) [NBP3-16922] - Western blot analysis of extracts of 293T cells, using ErbB3/Her3 Rabbit mAb (NBP3-16922) at 1:1000 dilution.293T cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 3min.

Applications for ErbB3/Her3 [p Tyr1222] Antibody (5Z1F0)

Application
Recommended Usage

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ErbB3/Her3

The ErbB3 gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. ErbB3 is a membrane-bound protein which has a neuregulin binding domain but not an active kinase domain. It can therefore bind this ligand but cannot convey a signal into the cell via protein phosphorylation. However it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers including prostate, bladder and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported but they have not been thoroughly characterized.

Long Name

Receptor Tyrosine Protein Kinase ErbB3

Alternate Names

HER3

Gene Symbol

ERBB3

Additional ErbB3/Her3 Products

Product Documents for ErbB3/Her3 [p Tyr1222] Antibody (5Z1F0)

Certificate of Analysis

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Product Specific Notices for ErbB3/Her3 [p Tyr1222] Antibody (5Z1F0)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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