ErbB4/Her4 Antibody (HFR-1) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-08902

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # HFR-1

Format

Azide and BSA Free
View all ErbB4/Her4 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human ErbB4/Her4 protein fragment (around aa 1116-1269) (exact sequence is proprietary) (Uniprot: Q15303)

Reactivity Notes

Predicted to work with Rat, Chicken.

Localization

Cytoplasmic, Cell Surface and Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07160).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for ErbB4/Her4 Antibody (HFR-1) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: ErbB4/Her4 Antibody (HFR-1) - Azide and BSA Free [NBP3-08902]

Immunocytochemistry/ Immunofluorescence: ErbB4/Her4 Antibody (HFR-1) - Azide and BSA Free [NBP3-08902]

Immunocytochemistry/Immunofluorescence: ErbB4/Her4 Antibody (HFR-1) - Azide and BSA Free [NBP3-08902] - Immunofluorescence Analysis of MCF-7 cells labeling HER-4 with ErbB4/Her4 Mouse Monoclonal Antibody (HFR-1) Goat anti- Mouse IgG-CF488 (Green). The nuclear counterstain is RedDot (Red).
Flow Cytometry: ErbB4/Her4 Antibody (HFR-1) - Azide and BSA Free [NBP3-08902]

Flow Cytometry: ErbB4/Her4 Antibody (HFR-1) - Azide and BSA Free [NBP3-08902]

Flow Cytometry: ErbB4/Her4 Antibody (HFR-1) - Azide and BSA Free [NBP3-08902] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using ErbB4/Her4 Mouse Monoclonal Antibody (HFR-1) followed by goat anti- Mouse IgG-CF488 (Blue); Isotype Control (Red).

Applications for ErbB4/Her4 Antibody (HFR-1) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: ErbB4/Her4

erbB4/HER4 is a receptor tyrosine kinase that belongs to the human epidermal growth factor receptor (EGFR) family. erbB4/HER4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain, and cerebellum (1). erbB4/HER4 is activated by neuregulins (NRG), betacellulin (BTC), and heparin-binding EGF-like growth factor (2). Neuregulins regulate the expression of ligand- and voltage-gated channels in neurons and skeletal muscle by the activation of ErbB 1-4. The erbB4/HER4 receptor is unique among these receptors because its C-terminal tail binds to a protein motif known as the PDZ domain (3).

Long Name

Receptor Tyrosine Protein Kinase ErbB4

Alternate Names

HER4

Gene Symbol

ERBB4

Additional ErbB4/Her4 Products

Product Documents for ErbB4/Her4 Antibody (HFR-1) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for ErbB4/Her4 Antibody (HFR-1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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