ERCC1 Antibody (ERCC1/2318)

Novus Biologicals | Catalog # NBP3-07161

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # ERCC1/2318
View all ERCC1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa 191-281) of human ERCC1 protein (exact sequence is proprietary) (Uniprot: P07992)

Localization

Nuclear

Marker

Tumor Progression Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08903)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for ERCC1 Antibody (ERCC1/2318)

Protein Array: ERCC1 Antibody (ERCC1/2318) [NBP3-07161]

Protein Array: ERCC1 Antibody (ERCC1/2318) [NBP3-07161]

Protein Array: ERCC1 Antibody (ERCC1/2318) [NBP3-07161] - Analysis of Protein Array containing more than 19,000 full-length human proteins using ERCC1 Mouse Monoclonal Antibody (ERCC1/2318).
ERCC1 Antibody (ERCC1/2318) Immunohistochemistry-Paraffin: ERCC1 Antibody (ERCC1/2318) [NBP3-07161] -

Immunohistochemistry-Paraffin: ERCC1 Antibody (ERCC1/2318) [NBP3-07161] -

Formalin-fixed, paraffin-embedded human breast cancer stained with ERCC1 Antibody (ERCC1/2318). HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.

Applications for ERCC1 Antibody (ERCC1/2318)

Application
Recommended Usage

Western Blot

1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA).
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: ERCC1

The mammalian ERCC1 (Excision Repair Cross Complementing) polypeptide is required for nucleotide excision repair (NER) of damaged DNA and is homologous to Saccharomyces cerevisiae RAD10, which functions in repair and mitotic intrachromosomal recombination. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells XPG cleaves 3' of the DNA lesion while the ERCC1 XPF complex makes the 5'incision. Elevated levels of ERCC1 have also been reported in Cisplatin resistant cells.

Long Name

Excision Repair Cross-complementing 1

Alternate Names

COFS4, DNA excision repair protein ERCC-1, excision repair cross-complementing rodent repair deficiency, complementationgroup 1 (includes overlapping antisense sequence), RAD10, UV20

Gene Symbol

ERCC1

UniProt

P07992 (Human)

Additional ERCC1 Products

Product Documents for ERCC1 Antibody (ERCC1/2318)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ERCC1 Antibody (ERCC1/2318)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

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