eRF1 Antibody (4F9H12) - BSA Free

Novus Biologicals | Catalog # NBP2-52552

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA, Flow Cytometry, Simple Western, CyTOF-ready

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4F9H12

Format

BSA Free
View all eRF1 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human eRF1 (AA: 288-437) expressed in E. Coli.

Reactivity Notes

Use in Human reported in scientific literature (PMID:33764477).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for eRF1 Antibody (4F9H12) - BSA Free

Western Blot: eRF1 Antibody (4F9H12)BSA Free [NBP2-52552]

Western Blot: eRF1 Antibody (4F9H12)BSA Free [NBP2-52552]

eRF1-Antibody-4F9H12-BSA-Free-Western-Blot-NBP2-52552-img0008.jpg
Flow Cytometry: eRF1 Antibody (4F9H12) - BSA Free [NBP2-52552]

Flow Cytometry: eRF1 Antibody (4F9H12) - BSA Free [NBP2-52552]

Flow Cytometry: eRF1 Antibody (4F9H12) - BSA Free [NBP2-52552] - Analysis of HepG2 cells using RF1 mouse mAb (green) and negative control (red).
Western Blot: eRF1 Antibody (4F9H12)BSA Free [NBP2-52552]

Western Blot: eRF1 Antibody (4F9H12)BSA Free [NBP2-52552]

Western Blot: eRF1 Antibody (4F9H12) - BSA Free [NBP2-52552] - Analysis using RF1 mAb against HEK293 (1) and RF1 (AA: 288-437)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: eRF1 Antibody (4F9H12)BSA Free [NBP2-52552]

Western Blot: eRF1 Antibody (4F9H12)BSA Free [NBP2-52552]

Western Blot: eRF1 Antibody (4F9H12) - BSA Free [NBP2-52552] - Analysis using RF1 mAb against human RF1 (AA: 288-437) recombinant protein. (Expected MW is 43 kDa)
Western Blot: eRF1 Antibody (4F9H12)BSA Free [NBP2-52552]

Western Blot: eRF1 Antibody (4F9H12)BSA Free [NBP2-52552]

Western Blot: eRF1 Antibody (4F9H12) - BSA Free [NBP2-52552] - Analysis using eRF1 mouse mAb against MCF-7 (1), T47D (2), MOLT4 (3), and Raji (4) cell lysate.
Flow Cytometry: eRF1 Antibody (4F9H12) - BSA Free [NBP2-52552]

Flow Cytometry: eRF1 Antibody (4F9H12) - BSA Free [NBP2-52552]

Flow Cytometry: eRF1 Antibody (4F9H12) - BSA Free [NBP2-52552] - Analysis of Hela cells using RF1 mouse mAb (green) and negative control (red).
ELISA: eRF1 Antibody (4F9H12) - BSA Free [NBP2-52552]

ELISA: eRF1 Antibody (4F9H12) - BSA Free [NBP2-52552]

ELISA: eRF1 Antibody (4F9H12) - BSA Free [NBP2-52552] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng).
eRF1 Antibody (4F9H12) - BSA Free

Simple Western: eRF1 Antibody (4F9H12) - BSA Free [NBP2-52552] -

Simple Western: eRF1 Antibody (4F9H12) - BSA Free [NBP2-52552] - Effect of CC-885 & CC-90009 on COL17A1 PTC readthrough. (A, B) HaCaT keratinocytes derived from an unaffected individual were exposed to various concentrations of CC-885 (A) or CC-90009 (B) with or without 100 μg/ml gentamicin sulfate for 48 h & cell viability was measured using the MTT assay in triplicate samples (±SD). Concentrations of CC-885 & CC-90009 are in nanomolar. (C, D) JEB01 keratinocytes derived from a JEB patient with a COL17A1 nonsense mutation (R688X/R688X) were exposed to the indicated concentrations of CC-885 (C) or CC-90009 (D) for 72 h & eRF3a, eRF3b & eRF1 levels were determined using automated capillary electrophoresis western analysis. GAPDH was used as loading control. (E, F) JEB01 keratinocytes were exposed to the indicated concentrations of CC-885 (E) or CC-90009 (F) in the presence or absence of 100 or 300 μg/ml gentamicin sulfate for 72 h & Collagen XVII levels were measured by SDS-PAGE followed by traditional western blotting. beta -actin was used as loading control. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33764477), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for eRF1 Antibody (4F9H12) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Simple Western

1:100

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.
See Simple Western Antibody Database for Simple Western validation: Tested in Caov-3, GM00798, HCT 116, HDQ-P1, NCI-H1299, SW900, separated by Size, antibody dilution of 1:100

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: eRF1

Termination of protein biosynthesis and release of the nascent polypeptide chain are signaled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome. The process of translation termination is universal and is mediated by protein release factors (RFs) and GTP. A class 1 RF recognizes the stop codon and promotes the hydrolysis of the ester bond linking the polypeptide chain with the peptidyl site tRNA, a reaction catalyzed at the peptidyl transferase center of the ribosome. Class 2 RFs, which are not codon specific and do not recognize codons, stimulate class 1 RF activity and confer GTP dependency upon the process. In prokaryotes, both class 1 RFs, RF1 and RF2, recognize UAA; however, UAG and UGA are decoded specifically by RF1 and RF2, respectively. In eukaryotes, eRF1, or ETF1, the functional counterpart of RF1 and RF2, functions as an omnipotent RF, decoding all 3 stop codons (Frolova et al., 1994 [PubMed 7990965]).[supplied by OMIM]

Alternate Names

D5S1995, ERF1eRF1, eukaryotic peptide chain release factor subunit 1, Eukaryotic release factor 1, eukaryotic translation termination factor 1, polypeptide chain release factor 1, Protein Cl1, RF1ERF, sup45 (yeast omnipotent suppressor 45) homolog-like 1, SUP45L1, TB3-1MGC111066

Gene Symbol

ETF1

Additional eRF1 Products

Product Documents for eRF1 Antibody (4F9H12) - BSA Free

Certificate of Analysis

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Product Specific Notices for eRF1 Antibody (4F9H12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for eRF1 Antibody (4F9H12) - BSA Free

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Protocols

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