ERK1/ERK2 Antibody (SR1700) - BSA Free

Novus Biologicals | Catalog # NBP3-21670

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1700 expressed in HEK293

Format

BSA Free
View all ERK1/ERK2 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human ERK1/ERK2 (Uniprot #: P27361, P28482)

Specificity

Detects endogenous levels of total ERK1/ERK2

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ERK1/ERK2 Antibody (SR1700) - BSA Free

Western Blot: ERK1/ERK2 Antibody (SR1700) [NBP3-21670] -

Western Blot: ERK1/ERK2 Antibody (SR1700) [NBP3-21670] - Western blot analysis using NBP3-21670 in HepG2 whole cell lysates.

Immunocytochemistry/Immunofluorescence: ERK1/ERK2 Antibody (SR1700) [NBP3-21670] -

Immunocytochemistry/Immunofluorescence: ERK1/ERK2 Antibody (SR1700) [NBP3-21670] - Immunofluorescent analysis of Hela cells, using NBP3-21670.

Applications for ERK1/ERK2 Antibody (SR1700) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:200

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunoprecipitation

1:50

Simple Western

1:10 - 1:250

Western Blot

1:500-1:2000
Application Notes
See Simple Western Antibody Database for Simple Western validation: Tested in PDLO lysate, separated by Size, antibody dilution of 1:10, 1:50, 1:250, apparent MW was 42 kDa

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ERK1/ERK2

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008]

Long Name

Extracellular Signal-regulated Kinase 1/2

Alternate Names

ERK1, ERK-1, ERT2, Extracellular signal-regulated kinase 1, extracellular signal-related kinase 1, HS44KDAP, HUMKER1A, Insulin-stimulated MAP2 kinase, MAPK 1, Microtubule-associated protein 2 kinase, mitogen-activated protein kinase 3, p44erk1, p44-ERK1, p44mapk, p44-MAPK, PRKM3

Gene Symbol

MAPK3

Additional ERK1/ERK2 Products

Product Documents for ERK1/ERK2 Antibody (SR1700) - BSA Free

Certificate of Analysis

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Product Specific Notices for ERK1/ERK2 Antibody (SR1700) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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