ERK5/BMK1 Antibody (OTI3H2)

Novus Biologicals | Catalog # NBP2-03371

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3H2
View all ERK5/BMK1 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human MAPK7 (NP_002740) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

88.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ERK5/BMK1 Antibody (OTI3H2)

Western Blot: ERK5 Antibody (3H2) [NBP2-03371] Analysis of extracts (35ug) from 9 different cell lines by using anti-ERK5 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: ERK5/BMK1 Antibody (OTI3H2) [NBP2-03371]

Immunocytochemistry/ Immunofluorescence: ERK5/BMK1 Antibody (OTI3H2) [NBP2-03371]

Immunocytochemistry/Immunofluorescence: ERK5 Antibody (3H2) [NBP2-03371] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ERK5.
Flow Cytometry: ERK5/BMK1 Antibody (OTI3H2) [NBP2-03371]

Flow Cytometry: ERK5/BMK1 Antibody (OTI3H2) [NBP2-03371]

Flow Cytometry: ERK5 Antibody (3H2) [NBP2-03371] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ERK5 antibody, and then analyzed by flow cytometry.
Western Blot: ERK5/BMK1 Antibody (OTI3H2) [NBP2-03371]

Western Blot: ERK5/BMK1 Antibody (OTI3H2) [NBP2-03371]

Western Blot: ERK5 Antibody (3H2) [NBP2-03371] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERK5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERK5.

Applications for ERK5/BMK1 Antibody (OTI3H2)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.6 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ERK5/BMK1

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is specifically activated by mitogen-activated protein kinase kinase 5 (MAP2K5/MEK5). It is involved in the downstream signaling processes of various receptor molecules including receptor type kinases, and G protein-coupled receptors. In response to extracelluar signals, this kinase translocates to cell nucleus, where it regulates gene expression by phosphorylating, and activating different transcription factors. Four alternatively spliced transcript variants of this gene encoding two distinct isoforms have been reported. [provided by RefSeq].

Long Name

Extracellular Signal-regulated Kinase 5

Alternate Names

BMK1, MAPK7

Entrez Gene IDs

5598 (Human)

Gene Symbol

MAPK7

Additional ERK5/BMK1 Products

Product Documents for ERK5/BMK1 Antibody (OTI3H2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ERK5/BMK1 Antibody (OTI3H2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
Intracellular Kinases

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Protocols

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