ERO1L Antibody - Azide Free

Novus Biologicals | Catalog # NB100-2525

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free
View all ERO1L alpha Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to the N-terminal region of human ERO1L [UniProt Q96HE7]

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34512174).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ERO1L Antibody - Azide Free

Western Blot: ERO1L Antibody [NB100-2525]

Western Blot: ERO1L Antibody [NB100-2525]

Western Blot: ERO1L Antibody [NB100-2525] - Analysis of ERO1L in 293 cell lysates.
Immunocytochemistry/ Immunofluorescence: ERO1L Antibody [NB100-2525]

Immunocytochemistry/ Immunofluorescence: ERO1L Antibody [NB100-2525]

Immunocytochemistry/Immunofluorescence: ERO1L Antibody [NB100-2525] - Detection of ERO1L in Hela cells.
Western Blot: ERO1L Antibody [NB100-2525]

Western Blot: ERO1L Antibody [NB100-2525]

Western Blot: ERO1L Antibody [NB100-2525] - Analysis of ERO1L in mouse white adipose tissue using anti-ERO1L antibody. Image from verified customer review.
Western Blot: ERO1L Antibody [NB100-2525]

Western Blot: ERO1L Antibody [NB100-2525]

ERO1L-Antibody-Western-Blot-NB100-2525-img0009.jpg
Western Blot: ERO1L Antibody [NB100-2525]

Western Blot: ERO1L Antibody [NB100-2525]

Western Blot: ERO1L Antibody [NB100-2525] - Detection of ERO1 alpha in an endogenous 293T cell lysate.

Applications for ERO1L Antibody - Azide Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:1000-1:2000
Application Notes
This ERO1L antibody is useful for Western Blot, where a band ~57 kDa is observed. Depending on the samples used ERO1L can also appear as a doublet which is believed to represent the oxidized and reduced forms of the protein. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 1 review rated 4 using NB100-2525 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 1.0% BSA

Format

Azide Free

Preservative

No Preservative

Concentration

2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ERO1L alpha

ERO1L (endoplasmic oxidoreductin-1-like protein) is localized in ER membrane as peripheral membrane protein on lumenal side, its association with ERP44 being essential for its retention in the ER, and is unique in coupleing oxygen reduction to de novo disulfides formation. ERO1L enzyme is broadly distributed at low levels in several tissues with highest levels in upper digestive tract especially the esophagus, and is stimulated by hypoxia via HIF-signaling. ERO1L generally exists as monomer, but has the ability to function as monomer or homodimer and interacts with PDILT. After translation, ERO1L gets n-glycosylated and the disulphide bonds constitute the redox-active center whereby the Cys-94/Cys-99 disulfide bond accept electron from P4HB followed by funneling the same to the active site disulfide Cys-394/Cys-397. ERO1L acts by oxidizing directly P4HB/PDI isomerase through direct disulfide exchange and can associates with ERP44. Its reoxidation probably involves electron transfer to molecular oxygen via FAD and acts independently of GSH. ERO1L is implicated in immunoglobulin folding, oxidative stress, release of unfolded cholera toxin from reduced P4HB/PDI upon Vibrio cholerae infection, and plays a key role in ER stress-induced CHOP-dependent apoptosis through inositol 1,4,5-trisphosphate receptor IP3R1 activation.

Long Name

Endoplasmic Reticulum Oxidoreductin 1 alpha

Alternate Names

ERO1-alpha, ERO1-L, ERO1-l alpha, ERO1-like, ERO1-like alpha, ERO1A, ERO1L

Gene Symbol

ERO1A

Additional ERO1L alpha Products

Product Documents for ERO1L Antibody - Azide Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ERO1L Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Redox Enzymes

Citations for ERO1L Antibody - Azide Free

Customer Reviews for ERO1L Antibody - Azide Free (1)

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Customer Images

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: white adipose tissue
    Species: Mouse
    Verified Customer | Posted 10/05/2015
    Ero1-La in western blot: mouse white adipose tissue
    ERO1L Antibody - Azide Free NB100-2525

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