ERP44 Antibody

Novus Biologicals | Catalog # NB100-93457

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all ERP44 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-EQKDSDNYRVFER corresponding to internal region according to NP_055866.1.

Epitope

C-EQKDSDNYRVFER

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ERP44 Antibody

Western Blot: ERP44 Antibody [NB100-93457]

Western Blot: ERP44 Antibody [NB100-93457]

Western Blot: ERP44 Antibody [NB100-93457] - Staining of K562 lysate with antibody at 0.2 ug/mL (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for ERP44 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

0.2 - 0.6 ug/mL
Application Notes
WB: Approx. 47 kDa band observed in K562 lysates (calculated MW of 47.0 kDa band according to NP_055866.1). An additional band of unknown identity was also consistently observed at 22 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ERP44

ERP44 mediates thiol-dependent retention in the early secretory pathway, forming mixed disulfides with substrate proteins through its conserved CRFS motif. Inhibits the calcium channel activity of ITPR1. May have a role in the control of oxidative protei

Alternate Names

endoplasmic reticulum protein 44, endoplasmic reticulum resident protein 44 kDa, ER protein 44, ERp44, KIAA0573endoplasmic reticulum resident protein 44, PDIA10, protein disulfide isomerase family A, member 10, thioredoxin domain containing 4 (endoplasmic reticulum), Thioredoxin domain-containing protein 4, TXNDC4

Entrez Gene IDs

23071 (Human); 76299 (Mouse); 298066 (Rat)

Gene Symbol

ERP44

UniProt

Q9BS26 (Human)

Additional ERP44 Products

Product Documents for ERP44 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ERP44 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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