Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 020 expressed in HEK293

Format

Azide and BSA Free
View all ERp72 Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human ERp72 (Uniprot#: P13667; Met1-Thr641).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for ERp72 Antibody (020) - Azide and BSA Free

Flow Cytometry: ERp72 Antibody (020) [NBP2-90208]

Flow Cytometry: ERp72 Antibody (020) [NBP2-90208]

Flow Cytometry: ERp72 Antibody (020) [NBP2-90208] - Flow cytometric analysis of Human PDIA4 expression on HepG2 cells. The cells were treated according to manufacturer's manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human PDIA4, t
Western Blot: ERp72 Antibody (020) [NBP2-90208]

Western Blot: ERp72 Antibody (020) [NBP2-90208]

Western Blot: ERp72 Antibody (020) [NBP2-90208] - Anti-PDIA4 rabbit monoclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lane B: NIH-3T3 Whole Cell Lysate. Lane C: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane.
Immunoprecipitation: ERp72 Antibody (020) [NBP2-90208]

Immunoprecipitation: ERp72 Antibody (020) [NBP2-90208]

Immunoprecipitation: ERp72 Antibody (020) [NBP2-90208] - PDIA4 was immunoprecipitated using: Lane A :0.5 mg Hela Whole Cell Lysate. Lane B:0.5 mg MCF-7 Whole Cell Lysate. 2 uL anti-PDIA4 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-PDIA4 rabbit monoclonal antibody,at 1:100 dilution. Secondary antibody:
ERp72 Antibody (020) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: ERp72 Antibody (020) [NBP2-90208] -

Immunocytochemistry/Immunofluorescence: ERp72 Antibody (020) [NBP2-90208] - Immunofluorescence staining of PDIA4 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP2-90208 antibody (dilution ratio 1:60) at 4? overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

Applications for ERp72 Antibody (020) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:25-1:100

Immunocytochemistry/ Immunofluorescence

1:20-1:100

Immunoprecipitation

1-4 ul/mg of lysate

Western Blot

1:500-1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ERp72

ERp72 is an endoplasmic reticulum luminal protein that is both a stress protein and a member of the protein disulfide isomerase family of proteins. Sequence analysis of ERp72 shows that it shares sequence identity with PDI (protein disulphide isomerase) at three discrete regions, as well as containing the ER retention signal KEEL at its C-terminus. ERp72 is shown to have three copies of sequence believed to be CGHC-containing active sites, which are also found in PDI. These CGHC sites are critical sites that are involved in the protein disulphide isomerization process. These results suggest that ERp72 is a newly described member of the family of CGHC-containing proteins that functions as a molecular chaperone. This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Alternate Names

Endoplasmic reticulum resident protein 70, Endoplasmic reticulum resident protein 72, ER protein 70, ERp70, ERP70ER protein 72, ERp72, ERp-72, ERP72EC 5.3.4.1, intestinal-related), protein disulfide isomerase family A, member 4, protein disulfide isomerase related protein (calcium-binding protein, protein disulfide isomerase-associated 4, protein disulfide-isomerase A4

Gene Symbol

PDIA4

Additional ERp72 Products

Product Documents for ERp72 Antibody (020) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for ERp72 Antibody (020) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ERp72 Antibody (020) - Azide and BSA Free

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Protocols

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FAQs

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