ERR gamma/NR3B3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32853

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Zebrafish

Predicted:

Chicken (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry Whole-Mount, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ERR gamma/NR3B3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human ERR gamma/NR3B3. The exact sequence is proprietary.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ERR gamma/NR3B3 Antibody - BSA Free

Western Blot: ERR gamma/NR3B3 Antibody [NBP1-32853]

Western Blot: ERR gamma/NR3B3 Antibody [NBP1-32853]

Western Blot: ERR gamma/NR3B3 Antibody [NBP1-32853] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ERR gamma antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: ERR gamma/NR3B3 Antibody [NBP1-32853]

Western Blot: ERR gamma/NR3B3 Antibody [NBP1-32853]

Western Blot: ERR gamma/NR3B3 Antibody [NBP1-32853] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE; antibody diluted at 1:1000.
Western Blot: ERR gamma/NR3B3 Antibody [NBP1-32853]

Western Blot: ERR gamma/NR3B3 Antibody [NBP1-32853]

Western Blot: ERR gamma/NR3B3 Antibody [NBP1-32853] - Rat spleen lysate/extract.
ERR gamma/NR3B3 Antibody

IHC-Wm- ERR gamma/NR3B3 Antibody [NBP1-32853] -

IHC-Wm- ERR gamma/NR3B3 Antibody [NBP1-32853] - 2 days-post-fertilization zebrafish embryo.ERR gamma antibody dilution: 1:200.
ERR gamma/NR3B3 Antibody

IHC-Wm-ERR gamma/NR3B3 Antibody [NBP1-32853] -

IHC-Wm-ERR gamma/NR3B3 Antibody [NBP1-32853] -2 days-post-fertilization zebrafish embryo.ERR gamma antibody dilution: 1:200.

Applications for ERR gamma/NR3B3 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry Whole-Mount

Assay dependent

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ERR gamma/NR3B3

Estrogen related receptor (ERR gamma), a NR3 Steroid Receptor is an orphan member of the nuclear hormone receptor superfamily. All the ERR family members (1,2, and 3) share an almost identical DNA binding domain, which has 68% amino acid identity with that of estrogen receptor. Studies involving the crystal structure of the LBD have shown a complex between ERR3 and SRC1. ERR gamma has been suggested to affect differentiation of the brain, heart, and kidney. ERR gamma binds as a monomer to an extended half site of the ERRE type (TCAAGGTCA). ERR gamma has been shown to interact with PGC1 alpha and has been implicated in the regulation of mitochondrial energy metabolism. In humans, ERR gamma pre mRNA undergoes extensive alternative splicing at the 5 prime end, yielding at least six mRNA splice variants and two protein isoforms that differ by 23 amino acids in the N terminus. ERR gamma has been shown to be over expressed in breast tumors, and its expression is correlated with levels of ErbB4, a likely indicator of preferred clinical course. As a result, ERR gamma has been suggested to be a potential biomarker for favorable clinical course and, possibly, hormonal sensitivity, and as a candidate target for therapeutic development.

Long Name

Estrogen-related Receptor gamma

Alternate Names

ERR3, ESRRG, NR3B3

Gene Symbol

ESRRG

Additional ERR gamma/NR3B3 Products

Product Documents for ERR gamma/NR3B3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ERR gamma/NR3B3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for ERR gamma/NR3B3 Antibody - BSA Free

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  • Q: I am looking for an antibody to detect human ERRgamma by Western blot in primary human muscle cells. There are several antibodies indicating for WB from Novus. Could you recommend the best one for me to order. thanks!

    A: NBP1-32853 works well for Western blot in human tissue. The positive control I would recommend is Hela, A431, HepG2 or Raji cells.

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