ERR gamma/NR3B3 Antibody - BSA Free

Novus Biologicals | Catalog # NLS5960

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken, Equine, Hamster, Monkey, Primate, Rabbit

Predicted:

Avian (100%), Bat (93%), Opossum (100%), Orangutan (100%), Xenopus (93%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ERR gamma/NR3B3 Primary Antibodies »

Product Specifications

Immunogen

Synthetic 15 amino acid peptide from C-terminus of humanERR gamma/NR3B3.

Epitope

C-Terminus

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Marmoset (100%).

Localization

Nuclear

Specificity

Human ERR gamma/NR3B3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Product can be stored undiluted at 4C for up to 1 month.

Scientific Data Images for ERR gamma/NR3B3 Antibody - BSA Free

Immunohistochemistry-Paraffin: ERR gamma/NR3B3 Antibody - BSA Free [NLS5960]

Immunohistochemistry-Paraffin: ERR gamma/NR3B3 Antibody - BSA Free [NLS5960]

Immunohistochemistry-Paraffin: ERR gamma/NR3B3 Antibody [NLS5960] - Lung, Non Small-Cell Carcinoma
Immunohistochemistry-Paraffin: ERR gamma/NR3B3 Antibody - BSA Free [NLS5960]

Immunohistochemistry-Paraffin: ERR gamma/NR3B3 Antibody - BSA Free [NLS5960]

Immunohistochemistry-Paraffin: ERR gamma/NR3B3 Antibody [NLS5960] - Analysis of Breast cancer using this antibody at 9 ug/ ml.
Immunohistochemistry-Paraffin: ERR gamma/NR3B3 Antibody - BSA Free [NLS5960]

Immunohistochemistry-Paraffin: ERR gamma/NR3B3 Antibody - BSA Free [NLS5960]

Immunohistochemistry-Paraffin: ERR gamma/NR3B3 Antibody [NLS5960] - Anti-ESRRG / ERR Gamma antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Immunohistochemistry-Paraffin: ERR gamma/NR3B3 Antibody - BSA Free [NLS5960]

Immunohistochemistry-Paraffin: ERR gamma/NR3B3 Antibody - BSA Free [NLS5960]

Immunohistochemistry-Paraffin: ERR gamma/NR3B3 Antibody [NLS5960] - Anti-ESRRG / ERR Gamma antibody IHC of human Ovary, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Immunohistochemistry-Paraffin: ERR gamma/NR3B3 Antibody - BSA Free [NLS5960]

Immunohistochemistry-Paraffin: ERR gamma/NR3B3 Antibody - BSA Free [NLS5960]

Immunohistochemistry-Paraffin: ERR gamma/NR3B3 Antibody [NLS5960] - Anti-ESRRG / ERR Gamma antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Immunohistochemistry-Paraffin: ERR gamma/NR3B3 Antibody - BSA Free [NLS5960]

Immunohistochemistry-Paraffin: ERR gamma/NR3B3 Antibody - BSA Free [NLS5960]

Immunohistochemistry-Paraffin: ERR gamma/NR3B3 Antibody [NLS5960] - Anti-ESRRG / ERR Gamma antibody IHC of human kidney, diabetes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Immunohistochemistry-Paraffin: ERR gamma/NR3B3 Antibody - BSA Free [NLS5960]

Immunohistochemistry-Paraffin: ERR gamma/NR3B3 Antibody - BSA Free [NLS5960]

Immunohistochemistry-Paraffin: ERR gamma/NR3B3 Antibody [NLS5960] - Breast, Carcinoma
Immunohistochemistry-Paraffin: ERR gamma/NR3B3 Antibody - BSA Free [NLS5960]

Immunohistochemistry-Paraffin: ERR gamma/NR3B3 Antibody - BSA Free [NLS5960]

Immunohistochemistry-Paraffin: ERR gamma/NR3B3 Antibody [NLS5960] - Human Brain, Glioblastoma tissue after heat-induced antigen retrieval.

Applications for ERR gamma/NR3B3 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10 - 1:500

Immunohistochemistry-Paraffin

9 - 17 ug/ml

Western Blot

1:100 - 1:2000
Application Notes
.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Background: ERR gamma/NR3B3

Estrogen receptor-related receptor (ERR gamma), a NR3 Steroid Receptor, has been suggested to affect differentiation of the brain, heart, and kidney. ERR gamma binds as a monomer to an extended half-site of the ERRE type (TCAAGGTCA). ERR gamma has been shown to interact with PGC-1 alpha and has been implicated in the regulation of mitochondrial energy metabolism. In humans, ERR gamma pre-mRNA undergoes extensive alternative splicing at the 5 end, yielding at least six mRNA splice variants and two protein isoforms that differ by 23 amino acids in the N terminus. ERR gamma has been shown to be overexpressed in breast tumors, and its expression is correlated with levels of ErbB4, a likely indicator of preferred clinical course. As a result, ERR gamma has been suggested to be a potential biomarker for favorable clinical course and, possibly, hormonal sensitivity, and as a candidate target for therapeutic development. ERR gamma expression at the mRNA and protein levels is isoform- and tissue-specific in humans. ERR gamma expression has been documented in human heart, brain, placenta, skeletal muscle, kidney, pancreas, spleen, prostate, testis, and small intestine. ESTs have been isolated from human tissue libraries, including cancerous fetus, uterus, lung, breast, head/neck, colon, skin, eye, adrenal, and breast, and normal placenta, kidney, brain, eye, pineal, ear, and heart.

Long Name

Estrogen-related Receptor gamma

Alternate Names

ERR3, ESRRG, NR3B3

Entrez Gene IDs

2104 (Human)

Gene Symbol

ESRRG

UniProt

P62508 (Human)

Additional ERR gamma/NR3B3 Products

Product Documents for ERR gamma/NR3B3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ERR gamma/NR3B3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

View specific protocols for ERR gamma/NR3B3 Antibody - BSA Free (NLS5960):

Immunohistochemistry Protocol for Estrogen Related Receptor gamma Antibody (NLS5960):
Immunohistochemistry

1. Prepare tissue with formalin fixation and by embedding it in paraffin wax.
2. Make 4 um sections and place on pre-cleaned and charged microscope slides.
3. Heat in a tissue-drying oven for 45 minutes @ 60 degrees Celcius.
4. Deparaffinize the tissues by wash drying the slides in 3 changes of xylene for 5 minutes each @ RT.
5. Rehydrate the tissues by washing the slides in 3 changes of 100% alcohol for 3 minutes each @ RT.
6. Wash the slides in 2 changes of 95% alcohol for 3 minutes each @ RT.
7. Wash the slides in 1 change of 80% alcohol for 3 minutes @ RT.
8. Rinse the slides in gentle running distilled water for 5 minutes @ RT.
9. Perform antigen retrieval by steaming the slides in 0.01M sodium citrate buffer (pH 6.0) @ 99-100 degrees Celcius
for 20 minutes.
10. Remove the slides from the heat and let stand in buffer @ RT for 20 minutes.
11. Rinse the slides in 1X TBS-T for 1 minute @ RT.

**Do not allow the tissues to dry at any time during the staining procedure**

12. Begin the immunostaining by applying a universal protein block for 20 minutes @ RT.
13. Drain protein block from the slides and apply the diluted primary antibody for 45 minutes @ RT.
14. Rinse the slide in 1X TBS-T for 1 minute @ RT.
15. Apply a biotinylated anti-rabbit IgG (H+L) secondary for 30 minutes @ RT.
16. Rinse the slide in 1X TBS-T for 1 minute @ RT.
17. Apply an alkaline phosphatase steptavidin for 30 minutes @ RT.
18. Rinse the slide in 1X TBS-T for 1 minute @ RT.
19. Apply an alkaline phosphatase chromagen substrate for 30 minutes @ RT.
20. Rinse the slide in distilled water for 1 minute @ RT.

**This method should only be used if the chromagen substrate is alcohol insoluble (ie: Vector Red, DAB)**

21. Dehydrate the tissue by washing the slides in 2 changes of 80% alcohol for 1 minute each @ RT.
22. Wash the slides in 2 changes of 95% alcohol for 1 minute each @ RT.
23. Wash the slides in 3 changes of 100% alcohol for 1 minute each @ RT.
24. Wash the slides in 3 changes of xylene for 1 minute each @ RT.
25. Apply cover slip.

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FAQs

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