Recombinant Porcine Erythropoietin/EPO Protein

Novus Biologicals | Catalog # NBP2-62447

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Key Product Details

Source

Pichia pastoris

Accession Number

P49157

Applications

Western Blot, ELISA
View all Erythropoietin/EPO Proteins and Enzymes »

Product Specifications

Description

Recombinant pig erythropoietin expressed in Pichia pastoris and purified by ion-exchange chromatography.

Source:Pichia pastoris

Amino Acid Sequence:18.6 kDa recombinant protein corresponding to aa 27 - 194 of native porcine erythropoietin (EPO).

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

18.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Porcine Erythropoietin/EPO Protein

Recombinant Porcine Erythropoietin/EPO Protein ELISA: Recombinant Porcine Erythropoietin/EPO Protein [NBP2-62447] -

ELISA: Recombinant Porcine Erythropoietin/EPO Protein [NBP2-62447] -

ELISA: Recombinant Porcine Erythropoietin/EPO Protein [NBP2-62447] - Pig Erythropoietin ELISA

Recombinant Pig erythropoietin detected using Rabbit anti Pig erythropoietin as the capture reagent and biotinylated Rabbit anti Pig erythropoietin as the detection reagent followed by Streptavidin:HRP.

Formulation, Preparation, and Storage

NBP2-62447
Preparation Method Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
Formulation PBS, 10% Trahalose
Preservative 10% Trehalose
Concentration Lyoph
Reconstitution Reconstitute with 20 ul sterile PBS containing 0.1% carrier protein. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix vial after reconstitution.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Erythropoietin/EPO

Erythropoietin (Epo) stimulates red blood cell production by docking with its cognate receptor on the erythroid progenitor cell and triggering an array of signaling pathways that inhibit apoptosis and promote cell proliferation and differentiation. In its pharmaceutical forms, epoetin and darbepoetin, Epo is widely used to treat various anemias, including those associated with cancer.

Alternate Names

ECYT5, EPO, MVCD2

Gene Symbol

EPO

UniProt

P49157 (Porcine)

Additional Erythropoietin/EPO Products

Product Documents for Recombinant Porcine Erythropoietin/EPO Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Porcine Erythropoietin/EPO Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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FAQs for Recombinant Porcine Erythropoietin/EPO Protein

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  • Q:

    Does the human Erythropoietin (Epo) polyclonal antibody (Catalog # AB-286-NA) bind Epo if the receptor is not present?

    A: AB-286-NA binds Epo if the receptor is not present.

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