ETHE1 Antibody (1621) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-43632
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 1621
Format
Azide and BSA Free
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Product Specifications
Immunogen
Full length human ETHE1 Recombinant protein.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for ETHE1 Antibody (1621) - Azide and BSA Free
Western Blot: ETHE1 Antibody (1621) [NBP2-43632]
Western Blot: ETHE1 Antibody (1621) [NBP2-43632] - Analysis of A. 30 ug PC-12 whole cell extract B. 30 ug Rat2 whole cell extract 12 % SDS-PAGE ETHE1 antibody [1621] dilution: 1:1000Western Blot: ETHE1 Antibody (1621) [NBP2-43632]
Western Blot: ETHE1 Antibody (1621) [NBP2-43632] - Analysis of A. 30 ug Neuro2A whole cell extract B. 30 ug GL261 whole cell extract C. 30 ug C8D30 whole cell extract D. 30 ug NIH-3T3 whole cell extract E. 30 ug Raw264.7 whole cell extract F. 30 ug C2C12 whole cell extract 12 % SDS-PAGE ETHE1 antibody [1621] dilution: 1:1000Immunocytochemistry/ Immunofluorescence: ETHE1 Antibody (1621) [NBP2-43632] -
ETHE1 antibody [GT1621] detects ETHE1 protein at mitochondria by immunofluorescent analysis.Sample: A431 cells were fixed in 2% paraformaldehyde/culture medium at 37C for 30 min.
Green: ETHE1 protein stained by ETHE1 antibody [GT1621] (NBP2-43632) diluted at 1:500.
Blue: Hoechst 33342 staining.
Applications for ETHE1 Antibody (1621) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: ETHE1
Alternate Names
D83198, EC 3.1.2.6, ethylmalonic encephalopathy 1, Ethylmalonic encephalopathy protein 1, Hepatoma subtracted clone one protein, HSCOEC 3.-, protein ETHE1, mitochondrial, YF13H12
Gene Symbol
ETHE1
Additional ETHE1 Products
Product Documents for ETHE1 Antibody (1621) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ETHE1 Antibody (1621) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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