Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Exosome component 1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 145 and 195 of human Exosome Component 1 using the numbering given in entry NP_057130.1 (GeneID 51013).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Exosome component 1 Antibody - BSA Free

Western Blot: Exosome component 1 Antibody [NBP2-22239]

Western Blot: Exosome component 1 Antibody [NBP2-22239]

Western Blot: Exosome component 1 Antibody [NBP2-22239] - Whole cell lysate (15 and 50 ug) from mouse NIH3T3 cells. NBP2-22239 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: Exosome component 1 Antibody [NBP2-22239]

Western Blot: Exosome component 1 Antibody [NBP2-22239]

Western Blot: Exosome component 1 Antibody [NBP2-22239] - Whole cell lysate from HeLa (15 and 50 ug), 293T (50 ug), and Jurkat (50 ug) cells. NBP2-22239 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: Exosome component 1 Antibody [NBP2-22239]

Immunoprecipitation: Exosome component 1 Antibody [NBP2-22239]

Immunoprecipitation: Exosome component 1 Antibody [NBP2-22239] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: NBP2-22239 used for IP at 6 ug/mg lysate. EXOSC1 was also immunoprecipitated by rabbit anti-EXOSC1 antibody. For blotting immunoprecipitated EXOSC1, NBP2-22239 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for Exosome component 1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg

Western Blot

1:1000 - 1:5000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Exosome component 1

p13 is a specific marker for human myeloid cells. Induction studies using HL-60 cells show that p13 is expressed during the early phases of myeloid differentiation. The mAb anti-p13 recognizes a nuclear antigen expressed in human granulocytes (98%) and monocytes (80%) residing in lymphoid and non-lymphoid tissues. BM3 is an early marker of myeloid differentiation. Immunoprecipitation experiments using S35 methionine labeled human myeloid leukemia cells show that BM3 identifies a 13 kDa protein.

Alternate Names

CGI-108,3'-5' exoribonuclease CSL4 homolog, CSL4 exosomal core protein homolog, Csl4p, CSL4p13, exosomal core protein CSL4, exosome component 1hCsl4p, homolog of yeast exosomal core protein CSL4, SKI4, Ski4p

Gene Symbol

EXOSC1

UniProt

NP_057130.1 (Human)

Additional Exosome component 1 Products

Product Documents for Exosome component 1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Exosome component 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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