EYA1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-58069

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (97%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all EYA1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: SQSPGQTGFLSYGTSFSTPQPGQAPYSYQMQGSSFTTSSGIYTGNNSLTNSSGFNSSQQDYPSYPSFGQGQYAQYYNSSPYPAHYMTSSNTSPTTPSTNATYQLQEPPSGITSQAVTDPTAEYSTIHSPSTPIKDSDSDRLRRGSD

Reactivity Notes

Mouse reactivity reported from a verified customer review.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EYA1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: EYA1 Antibody [NBP2-58069]

Immunocytochemistry/ Immunofluorescence: EYA1 Antibody [NBP2-58069]

Immunocytochemistry/Immunofluorescence: EYA1 Antibody [NBP2-58069] - Staining of human cell line RH-30 shows localization to nucleoplasm & nuclear bodies. Antibody staining is shown in green.
Immunohistochemistry-Frozen: EYA1 Antibody [NBP2-58069]

Immunohistochemistry-Frozen: EYA1 Antibody [NBP2-58069]

Immunohistochemistry-Frozen: EYA1 Antibody [NBP2-58069] - The staining was done on an E11.5 mouse transverse section (fixed on 4% PFA overnight) and done using standard IF techniques. Antibody at 1:50. Blocking: 1 hour with 1% BSA before addition of the primary antibody. Secondary antibody: Alexa Fluor Donkey Anti Rabbit 488.

Applications for EYA1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25 - 2 ug/mL

Immunohistochemistry-Frozen

EYA1 Antibody is validated for IHC-Fr from a verified customer review.
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 5 using NBP2-58069 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EYA1

The EYA1 gene encodes an eyes absent homolog 1 protein that exists in three isoforms: isoform 1: 592 amino acids long, 64 kDA; isoform 2: 559 amino acids long, 61 kDA; and isoform 3: 557 amino acids long; 60 kDA. The protein coded by the EYA1 gene functions in the development of the kidney, brancial arches, eyes, and ears. Mutations in the EYA1 gene lead to branchiootorenal dysplasia syndrome, congenital cataracts and ocular anterior segment anomalies, and branchiootic syndrome. The EYA1 gene is also linked to fraser syndrome, microphthalmia, cataracts, enlarged vestibular aqueducts, townes-brocks syndrome, and anophthalmia.

Alternate Names

BOP, BOR, EC 3.1.3.48, eyes absent (Drosophila) homolog 1, eyes absent homolog 1, eyes absent homolog 1 (Drosophila), MGC141875

Gene Symbol

EYA1

Additional EYA1 Products

Product Documents for EYA1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for EYA1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for EYA1 Antibody - BSA Free (1)

5 out of 5
1 Customer Rating
5 Stars
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4 Stars
0%
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1 Stars
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Customer Images

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  • EYA1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: E11.5 mouse embryo fixed in 4% PFA
    Species: Mouse
    Verified Customer | Posted 02/12/2021
    Eya expression in a transverse section of an E11.5 mouse embryo
    Dilution used - 1:50. The staining was done on an E11.5 mouse transverse section (fixed on 4% PFA overnight) and done using standard IF techniques. Blocking - 1 hour with 1% BSA before addition of the primary antibody Secondary antibody - Alexa Fluor Donkey Anti Rabbit 488
    EYA1 Antibody - BSA Free NBP2-58069
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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Protocols

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