Factor XII heavy chain Antibody (3A3) - Azide and BSA Free

Novus Biologicals | Catalog # H00002161-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3A3

Format

Azide and BSA Free
View all Factor XII heavy chain Primary Antibodies »

Product Specifications

Immunogen

F12 (AAH12390, 191 a.a. ~ 300 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GHQFEGAEEYASFLQEAQVPFLSLERCSAPDVHGSSILPGMLCAGFLEGGTDACQGDSGGPLVCEDQAAERRLTLQGIISWGSGCGDRNKPGVYTDVAYYLAWIREHTVS

Specificity

F12 - coagulation factor XII (Hageman factor)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Factor XII heavy chain Antibody (3A3) - Azide and BSA Free

Proximity Ligation Assay: Factor XII heavy chain Antibody (3A3) [H00002161-M01]

Proximity Ligation Assay: Factor XII heavy chain Antibody (3A3) [H00002161-M01]

Proximity Ligation Assay: Factor XII heavy chain Antibody (3A3) [H00002161-M01] - Analysis of protein-protein interactions between GP1BA and F12. HeLa cells were stained with anti-GP1BA rabbit purified polyclonal 1:1200 and anti-F12 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Sandwich ELISA: Factor XII heavy chain Antibody (3A3) [H00002161-M01] - Detection limit for recombinant GST tagged F12 is approximately 0.03ng/ml as a capture antibody.

Applications for Factor XII heavy chain Antibody (3A3) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Factor XII heavy chain

This gene encodes coagulation factor XII which circulates in blood as a zymogen. This single chain zymogen is converted to a two-chain serine protease with an heavy chain (alpha-factor XIIa) and a light chain. The heavy chain contains two fibronectin-type domains, two epidermal growth factor (EGF)-like domains, a kringle domain and a proline-rich domain, whereas the light chain contains only a catalytic domain. On activation, further cleavages takes place in the heavy chain, resulting in the production of beta-factor XIIa light chain and the alpha-factor XIIa light chain becomes beta-factor XIIa heavy chain. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then to beta-factor XIIa. The active factor XIIa participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. It activates coagulation factors VII and XI. Defects in this gene do not cause any clinical symptoms and the sole effect is that whole-blood clotting time is prolonged.

Alternate Names

beta-factor XIIa part 1, coagulation factor XII, coagulation factor XII (Hageman factor), coagulation factor XIIa heavy chain, coagulation factor XIIa light chain, EC 3.4.21, EC 3.4.21.38, HAE3, HAEX, HAFbeta-factor XIIa part 2, Hageman factor

Entrez Gene IDs

2161 (Human)

Gene Symbol

F12

OMIM

234000 (Human)

UniProt

AAH12390 (Human)

Additional Factor XII heavy chain Products

Product Documents for Factor XII heavy chain Antibody (3A3) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Factor XII heavy chain Antibody (3A3) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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