FADD Antibody (10L6M0)

Novus Biologicals | Catalog # NBP3-15951

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 10L6M0 expressed in HEK293
View all FADD Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-205 of mouse FADD (NP_034305.1). MDPFLVLLHSLSGSLSGNDLMELKFLCRERVSKRKLERVQSGLDLFTVLLEQNDLERGHTGLLRELLASLRRHDLLQRLDDFEAGTATAAPPGEADLQVAFDIVCDNVGRDWKRLARELKVSEAKMDGIEEKYPRSLSERVRESLKVWKNAEKKNASVAGLVKALRTCRLNLVADLVEEAQESVSKSENMSPVLRDSTVSSSETP

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FADD Antibody (10L6M0)

FADD Antibody (10L6M0)

Western Blot: FADD Antibody (10L6M0) [FADD] -

Western Blot: FADD Antibody (10L6M0) [FADD] - Western blot analysis of various lysates using FADD Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 180s.
FADD Antibody (10L6M0)

Immunohistochemistry: FADD Antibody (10L6M0) [FADD] -

Immunohistochemistry: FADD Antibody (10L6M0) [FADD] - Immunohistochemistry analysis of paraffin-embedded human lung using FADD Rabbit mAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
FADD Antibody (10L6M0)

Immunohistochemistry: FADD Antibody (10L6M0) [FADD] -

Immunohistochemistry: FADD Antibody (10L6M0) [FADD] - Immunohistochemistry analysis of paraffin-embedded rat lung using FADD Rabbit mAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
FADD Antibody (10L6M0)

Immunohistochemistry: FADD Antibody (10L6M0) [FADD] -

Immunohistochemistry: FADD Antibody (10L6M0) [FADD] - Immunohistochemistry analysis of paraffin-embedded human colon using FADD Rabbit mAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
FADD Antibody (10L6M0)

Western Blot: FADD Antibody (10L6M0) [FADD] -

Western Blot: FADD Antibody (10L6M0) [FADD] - Western blot analysis of various lysates using FADD Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
FADD Antibody (10L6M0)

Immunohistochemistry: FADD Antibody (10L6M0) [FADD] -

Immunohistochemistry: FADD Antibody (10L6M0) [FADD] - Immunohistochemistry analysis of paraffin-embedded mouse spleen using FADD Rabbit mAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Applications for FADD Antibody (10L6M0)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FADD

Fas-associating protein with death domain (FADD) is an adaptor protein involved in the apoptosis induced by Fas and TNF-R1, and mitogen induced cell proliferation (1). FADD consists of death domain (DD) at the C-terminal and death effector domain (DED) at the N-terminal (2). DD interacts with Fas and TNF-R1, which exposes the DED portion on the FADD. Unmasked DED binds to the DED of pro-caspase-8, which activates the cysteine protease cascade (3). The development of autoimmune lymphoproliferative (lpr) diseases has been linked to inactivation of Fas-FADD interaction (4). Also, the deficiency in FADD can inhibit TRAIL-induced cell apoptosis.

Long Name

Fas-associated Death Domain Protein

Alternate Names

MORT1

Gene Symbol

FADD

Additional FADD Products

Product Documents for FADD Antibody (10L6M0)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for FADD Antibody (10L6M0)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for FADD Antibody (10L6M0)

There are currently no reviews for this product. Be the first to review FADD Antibody (10L6M0) and earn rewards!

Have you used FADD Antibody (10L6M0)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies

Associated Pathways

Apoptosis Signaling Pathway Apoptosis Signaling Pathway Thumbnail