FAM134B Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-55248

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all FAM134B Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: ENGMGTNDEDELSLGLPTELKRKKEQLDSGHRPSKETQSAAGLTLPLNSDQTFHLMSNLAGDVITAAVTAAIKDQLEGVQQALSQAAPIPEEDTDTEEGDDFELLDQSELDQIESELGLTQDQEAEAQQNKKSSG

Reactivity Notes

Mouse 86%, Rat 84%

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FAM134B Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: FAM134B Antibody [NBP2-55248]

Immunocytochemistry/ Immunofluorescence: FAM134B Antibody [NBP2-55248]

Immunocytochemistry/Immunofluorescence: FAM134B Antibody [NBP2-55248] - Staining of human cell line HeLa shows localization to nuclear bodies & endoplasmic reticulum. Antibody staining is shown in green.
FAM134B Antibody

Western Blot: FAM134B Antibody [NBP2-55248] -

Western Blot: FAM134B Antibody [NBP2-55248] - Palmitate inhibits EBSS-induced ER-phagy. a–c Cells transiently expressing mRFP-LC3 were starved in EBSS for 3 h in the presence or absence of palmitate (0.1 mM) & were co-treated for 3 h with Baf A1. Treated cells were stained for the ER marker KDEL. Representative micrographs (a) & quantification (b) of colocalization between mRFP-LC3 & KDEL (n = 10 per each group). Scale bar, 10 μm. Data are mean ± SEM; ****p < 0.0001 vs. control, ###p = 0.0002, ####p < 0.0001, ††††p < 0.0001. c The ratio of the average number of KDEL-positive mRFP-LC3 puncta to the total number of mRFP-LC3 puncta per cell (n = 10 per each group). Data are mean ± SEM; ****p < 0.0001 vs. control, ####p < 0.0001, ††††p < 0.0001. d & e Immunoblotting analysis (d) & quantification (e) of ER-phagy receptors & LC3-II in cells starved in EBSS for 3 h in the presence or absence of palmitate (0.1 mM) & were co-treated for 3 h with Baf A1 (FAM134B; n = 8 for control, PA, EBSS, PA + EBSS, n = 5 for control + Baf A1, PA + Baf A1, EBSS + Baf A1, PA + EBSS + Baf A1, RTN3; n = 4 per each group, CCPG1; n = 3 per each group, LC3-II; n = 9 for control, PA, EBSS, PA + EBSS, n = 4 for control + Baf A1, PA + Baf A1, EBSS + Baf A1, PA + EBSS + Baf A1). Data are mean ± SEM; FAM134B; ****p < 0.0001 vs. control, ##p = 0.0016, ††p = 0.0094, CCPG1; ****p < 0.0001 vs. control, LC3-II; *p = 0.0331, ****p < 0.0001 vs. control, ###p = 0.0002 at PA vs. PA + Baf A1, ###p = 0.0005 at PA + EBSS vs. PA + EBSS + Baf A1, ####p < 0.0001, †p = 0.0203. n.s., no significant difference Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33823883), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
FAM134B Antibody

Western Blot: FAM134B Antibody [NBP2-55248] -

Western Blot: FAM134B Antibody [NBP2-55248] - Palmitate impairs ER-phagy. a & b Immunoblotting analysis (a) & quantification (b) of ER-phagy receptors (FAM134B, RTN3, & CCPG1) in cells treated with 0.1 mM palmitate for the indicated times (FAM134B & RTN3; n = 4 per each group, CCPG1; n = 3 per each group). Data are mean ± SEM; FAM134B; **p = 0.0053, ***p = 0.0005 vs. control, CCPG1; *p = 0.0388, ***p = 0.0004 vs. control. c & d Cells transiently expressing mRFP-LC3 were treated with 0.1 mM palmitate for the indicated times in the presence or absence of Baf A1 (200 nM). Treated cells were stained for the ER marker KDEL. Representative micrographs (c) & quantification (d) of colocalization between mRFP-LC3 & KDEL (n = 13 per each group). Scale bar, 10 μm. Data are mean ± SEM; **p = 0.0029, ***p = 0.0008 vs. control, ##p = 0.0025. n.s., no significant difference Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33823883), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for FAM134B Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 5 using NBP2-55248 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FAM134B

FAM134B is required for long-term survival of nociceptive and autonomic ganglion neurons

Alternate Names

FAM134B protein, family with sequence similarity 134, member B, FLJ20152, FLJ22155, FLJ22179, HSAN2B, JK1

Gene Symbol

RETREG1

Additional FAM134B Products

Product Documents for FAM134B Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for FAM134B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for FAM134B Antibody - BSA Free

Customer Reviews for FAM134B Antibody - BSA Free (1)

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  • FAM134B Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HEK293 cell lysate
    Species: Mouse and Human
    Verified Customer | Posted 05/12/2017
    1: HEK293T 2: HEK293T mouse FAM134B over-expression, 1 μg 3: HEK293T mouse FAM134B over-expression, 4 μg 4: HEK293T human FAM134B over-expression, 1 μg 5: HEK293T human FAM134B over-expression, 4 μg
    FAM134B Antibody - BSA Free NBP2-55248

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