FANCF Antibody

Novus Biologicals | Catalog # NBP1-52057

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all FANCF Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-RPQEELEPGIHK corresponding to internal region according to NP_073562.1.

Epitope

C-RPQEELEPGIHK

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for FANCF Antibody

FANCF Antibody

Western Blot: FANCF Antibody [NBP1-52057] -

Western Blot: FANCF Antibody [NBP1-52057] - HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human FANCF with C-terminal MYC tag probed with NBP1-52057 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with NBP1-52057 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.

Applications for FANCF Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

0.2 - 1 ug/ml
Application Notes
WB: In transfected HEK293 transiently expressing full-length human FANCF (myc and DYKDDDDK tagged), a band of approx. 40 kDa band was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FANCF

The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group F. [provided by RefSeq]

Alternate Names

FAF, Fanconi anemia group F protein, Fanconi anemia, complementation group F, MGC126856, Protein FACF

Entrez Gene IDs

2188 (Human)

Gene Symbol

FANCF

UniProt

NP_073562.1 (Human)

Additional FANCF Products

Product Documents for FANCF Antibody

Certificate of Analysis

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Product Specific Notices for FANCF Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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