FARP1 Antibody

Novus Biologicals | Catalog # NBP2-76803

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all FARP1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence GEIEQRPTPGSRL-C, from the N Terminus of the protein sequence according to NP_005757.1; NP_001001715.2; NP_001273768.1.

Specificity

This antibody is expected to recognize isoform 1 (NP_005757.1), isoform 2 (NP_001001715.2), and isoform 3 (NP_001273768.1)

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for FARP1 Antibody

Flow Cytometry: FARP1 Antibody [NBP2-76803]

Flow Cytometry: FARP1 Antibody [NBP2-76803]

Flow Cytometry: FARP1 Antibody - Analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (1:50 dilution) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunofluorescence: FARP1 Antibody [NBP2-76803]

Immunofluorescence: FARP1 Antibody [NBP2-76803]

Immunofluorescence: FARP1 Antibody - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (1:50 dilution) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).
Immunofluorescence: FARP1 Antibody [NBP2-76803]

Immunofluorescence: FARP1 Antibody [NBP2-76803]

Immunofluorescence: FARP1 Antibody [NBP2-76803] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (1:50 dilution) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Applications for FARP1 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/ml

Peptide ELISA

Detection Limit 1:32000
Application Notes
WB: Preliminary experiments in human liver, kidney and lung lysates gave no specific signal but low background.

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 degrees C. Avoid freeze/thaw cycles.

Background: FARP1

This gene was originally isolated through subtractive hybridization due to its increased expression in differentiated chondrocytes versus dedifferentiated chondrocytes. The resulting protein contains a predicted ezrin-like domain, a Dbl homology domain, and a pleckstrin homology domain. It is believed to be a member of the band 4.1 superfamily whose members link the cytoskeleton to the cell membrane. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Long Name

FERM, ARHGEF and pleckstrin domain-containing protein 1

Alternate Names

CDEP, PLEKHC2

Entrez Gene IDs

10160 (Human)

Gene Symbol

FARP1

UniProt

NP_005757 (Human), NP_001273768.1 (Human), NP_001001715.2 (Human)

Additional FARP1 Products

Product Documents for FARP1 Antibody

Certificate of Analysis

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Product Specific Notices for FARP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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