Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 kappa Clone # H22

Format

Azide and BSA Free
View all Fc gamma RI/CD64 Primary Antibodies »

Product Specifications

Immunogen

This recombinant antibody was prepared by cloning the single chain fragment (scFv) derived from the murine anti-human Fc gamma RI/CD64 mAb m22 into the bacterial expression vector pBM1.1, and fusing it to a deletion mutant of Pseudomonas exotoxin A.

Specificity

This antibody is specific for human Fc gamma RI/CD64, and specifically an epitope outside the Fc binding domain. The binding activity of the VH/VL antibody format was unaffected by fusion of the m22(scFv) coding regions to the truncated ETA coding sequences

Clonality

Monoclonal

Host

Human

Isotype

IgG1 kappa

Description

Novus Biologicals Human Fc gamma RI/CD64 Antibody (H22) - Chimeric - Azide and BSA Free (NBP2-81097) is a recombinant monoclonal antibody validated for use in ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Applications for Fc gamma RI/CD64 Antibody (H22) - Chimeric - Azide and BSA Free

Application
Recommended Usage

ELISA

Validated for Competitive ELISA
Application Notes
This chimeric human antibody was made using the variable domain sequences of the original IgG1 format, for improved compatibility with existing reagents, assays and techniques.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: Fc gamma RI/CD64

CD64 is a 72 kD single chain type I glycoprotein also known as Fc gammaRI and FcR I. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes. The expression can be upregulated by IFN- gamma stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).

Long Name

Fc gamma Receptor I

Alternate Names

CD64, CD64a, FCGR1, FcgRI, FcgRIA, FCRI, FcRIA

Gene Symbol

FCGR1A

Additional Fc gamma RI/CD64 Products

Product Documents for Fc gamma RI/CD64 Antibody (H22) - Chimeric - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Fc gamma RI/CD64 Antibody (H22) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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