Fc gamma RII/RIII (CD32/CD16) Antibody (2.4G2) [FITC]

Novus Biologicals | Catalog # NBP2-52644F

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Block/Neutralize, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # 2.4G2
View all Fc gamma RII/RIII (CD32/CD16) Primary Antibodies »

Product Specifications

Immunogen

Mouse BALB/c Macrophage J774 Cell Line.

Reactivity Notes

Mouse

Specificity

This antibody binds to an epitope on the extracellular domains of the mouse Fc(gamma)III and Fc(gamma)II, thereby inhibiting their ability to bind antibodies via their Fc region. receptors. Also binding the Fc(gamma)I receptor (CD64) via its Fc domain.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Applications for Fc gamma RII/RIII (CD32/CD16) Antibody (2.4G2) [FITC]

Application
Recommended Usage

Block/Neutralize

Optimal dilutions of this antibody should be experimentally determined.

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Spectra Viewer

Plan Your Experiments

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Fc gamma RII/RIII (CD32/CD16)

Fc gamma RII/CD32 and Fc gamma RIII/CD16 proteins are low/intermediate affinity receptors for polyvalent immune-complexed IgG. Fc gamma RII is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. Fc gamma RIIA associates with FcR gamma and delivers an activating signal through its cytoplasmic ITAM motif. In contrast, Fc gamma RIIB delivers an inhibitory signal through its ITIM motif. Fc gamma RIIC represents an unequal cross-over event between the IIA and IIB genes. Fc gamma RIII is expressed on NK cells, T cells, monocytes, and macrophages. It is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody-dependent cytotoxicity, and clearance of immune complexes. 

 

Long Name

Fc gamma Receptor II/III

Alternate Names

CD32/CD16

Gene Symbol

FCGR3A

Additional Fc gamma RII/RIII (CD32/CD16) Products

Product Documents for Fc gamma RII/RIII (CD32/CD16) Antibody (2.4G2) [FITC]

Certificate of Analysis

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Product Specific Notices for Fc gamma RII/RIII (CD32/CD16) Antibody (2.4G2) [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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