FEN-1 Antibody - BSA Free

Western Blot: FEN-1 Antibody - BSA Free [NB100-320] -

Expression of Fen1 in the mitochondria of differentiated cells. (A) Representative Western blots for mitochondrial cell extracts of Prolif and Diff cells. (B) Fen1 protein signal was normalized to that for Complex 1 as a loading control for mitochondria, with laminin as a marker for the nucleus. Mitochondrial Fen1 levels were divided by whole-cell Fen1 levels to calculate mitochondrial Fen1 per cell. Error bars indicate the standard deviation (n = 3). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37628896), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: FEN-1 Antibody - BSA Free [NB100-320] -

Flap excision activity in proliferating and differentiated CAD cells. (A) Substrate for the flap endonuclease assay. The double-flap structure is optimal for Fen1 [27]; the target strand for that enzyme is labeled on its 5′ end the TAMRA fluor. The expected Fen1 product is shown. (B) Representative gel for the flap excision assay using whole-cell extracts from Prolif and Diff cells. Increasing amounts of extract were used for 1 pmol of substrate; Fen1 (51 nmol) was used as a positive control. The incubation was performed at 37 C for 30 min. (C) Quantification using Image J (version 2.0.0-rc-68/1.52i) (n = 3). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37628896), licensed under a CC-BY license. Not internally tested by Novus Biologicals.