Ferritin Light Chain Antibody (JM10-37)
Novus Biologicals | Catalog # NBP2-66836
Recombinant Monoclonal Antibody
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JM10-37 expressed in HEK293
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Product Specifications
Immunogen
Synthetic peptide within Human Ferritin Light Chain aa 126-175 / 175. (SwissProt: P02792 Human; SwissProt: P29391 Mouse; SwissProt: P02793 Rat)
Localization
Cytosol, extracellular exosome, extracellular region, autolysosome, azurophil granule lumen, cytoplasm, intracellular ferritin complex, membrane.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Ferritin Light Chain Antibody (JM10-37)
Western Blot: Ferritin Light Chain Antibody (JM10-37) [NBP2-66836]
Western Blot: Ferritin Light Chain Antibody (JM10-37) [NBP2-66836] - Analysis of Ferritin Light Chain on rat liver tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. APPLICATIONSImmunohistochemistry-Paraffin: Ferritin Light Chain Antibody (JM10-37) [NBP2-66836]
Immunohistochemistry-Paraffin: Ferritin Light Chain Antibody (JM10-37) [NBP2-66836] - Analysis of paraffin-embedded mouse testes tissue using anti-Ferritin Light Chain antibody. Counter stained with hematoxylin.Immunohistochemistry-Paraffin: Ferritin Light Chain Antibody (JM10-37) [NBP2-66836]
Immunohistochemistry-Paraffin: Ferritin Light Chain Antibody (JM10-37) [NBP2-66836] - Analysis of paraffin-embedded human brain tissue using anti-Ferritin Light Chain antibody. Counter stained with hematoxylin.Immunohistochemistry-Paraffin: Ferritin Light Chain Antibody (JM10-37) [NBP2-66836]
Immunohistochemistry-Paraffin: Ferritin Light Chain Antibody (JM10-37) [NBP2-66836] - Analysis of paraffin-embedded human kidney tissue using anti-Ferritin Light Chain antibody. Counter stained with hematoxylin.Immunohistochemistry-Paraffin: Ferritin Light Chain Antibody (JM10-37) [NBP2-66836]
Immunohistochemistry-Paraffin: Ferritin Light Chain Antibody (JM10-37) [NBP2-66836] - Analysis of paraffin-embedded human liver tissue using anti-Ferritin Light Chain antibody. Counter stained with hematoxylin.Immunohistochemistry-Paraffin: Ferritin Light Chain Antibody (JM10-37) [NBP2-66836]
Immunohistochemistry-Paraffin: Ferritin Light Chain Antibody (JM10-37) [NBP2-66836] - Analysis of paraffin-embedded mouse kidney tissue using anti-Ferritin Light Chain antibody. Counter stained with hematoxylin.Applications for Ferritin Light Chain Antibody (JM10-37)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:100-1:500
Western Blot
1:1000-1:5000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Ferritin Light Chain
Alternate Names
Ferritin L subunit, ferritin L-chain, ferritin light chain, ferritin light polypeptide-like 3, ferritin, light polypeptide, MGC71996, NBIA3
Gene Symbol
FTL
Additional Ferritin Light Chain Products
Product Documents for Ferritin Light Chain Antibody (JM10-37)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Ferritin Light Chain Antibody (JM10-37)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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