Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry

Label

Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)

Antibody Source

Polyclonal Sheep IgG
View all Fetal Hemoglobin Primary Antibodies »

Product Specifications

Immunogen

Purified human hemoglobin F of fetal cord blood cell origin.

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with human hemoglobin F. No antibody was detected against hemoglobin A1, A2, S or human serum proteins.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Fetal Hemoglobin Antibody [Alexa Fluor® 647]

Fetal Hemoglobin Antibody [Alexa Fluor® 647]

Flow Cytometry: Sheep Fetal Hemoglobin pAb [Alexa Fluor® 647] [NB110-41084AF647]

Flow Cytometry: Sheep Fetal Hemoglobin pAb [Alexa Fluor® 647] [NB110-41084AF647] - HbF cells in mouse. These mice are under stressed erythropoiesis. This antibody used to detect fetal hemoglobin in RBC. RBC was fixed by paraformaldehyde followed by permeabilization with 0.01% triton. These cells were incubated with antibody at 1:500 dilution for 30-60 min at room temperature. Image from a verified customer review.

Applications for Fetal Hemoglobin Antibody [Alexa Fluor® 647]

Application
Recommended Usage

Flow Cytometry

Validated for Flow from a verified customer review.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NB110-41084AF647 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Fetal Hemoglobin

The gamma globin genes (HBG1 and HBG2) are normally expressed in the fetal liver, spleen and bone marrow. Two gamma chains together with two alpha chains constitute fetal hemoglobin (HbF) which is normally replaced by adult hemoglobin (HbA) at birth. In some beta-thalassemias and related conditions, gamma chain production continues into adulthood. The two types of gamma chains differ at residue 136 where glycine is found in the G-gamma product (HBG2) and alanine is found in the A-gamma product (HBG1). The former is predominant at birth. The order of the genes in the beta-globin cluster is: 5'- epsilon -- gamma-G -- gamma-A -- delta -- beta--3'.

Alternate Names

abnormal hemoglobin, A-gamma globin, gamma A hemoglobin, gamma globin, gamma-1-globin, gamma-2-globin, gamma-globin chain, G-gamma globin Paulinia, Hb F Agamma, hb F Ggamma, HBGA, HBGR, HBG-T1, HBG-T2, Hemoglobin gamma-1 chain, hemoglobin gamma-2 chain, Hemoglobin gamma-A chain, hemoglobin gamma-G chain, hemoglobin subunit gamma-1, hemoglobin subunit gamma-2, hemoglobin, gamma A, hemoglobin, gamma, regulator of, HSGGL1, methemoglobin, PRO2979

Gene Symbol

HBG1

Additional Fetal Hemoglobin Products

Product Documents for Fetal Hemoglobin Antibody [Alexa Fluor® 647]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Fetal Hemoglobin Antibody [Alexa Fluor® 647]



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Fetal Hemoglobin Antibody [Alexa Fluor® 647] (1)

5 out of 5
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  • Fetal Hemoglobin Antibody [Alexa Fluor® 647]
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Whole blood
    Species: Mouse
    Verified Customer | Posted 05/12/2025
    HbF cells in mouse.
    These mice are under stressed erythropoiesis. This antibody used to detect fetal hemoglobin in RBC. RBC was fixed by paraformaldehyde followed by permeabilization with 0.01% triton. These cells were incubated with antibody at 1:500 dilution for 30-60 min at room temperature.
    Fetal Hemoglobin Antibody [Alexa Fluor® 647] NB110-41084AF647
    Bio-Techne Response
    This review reflects a new species or application tested on a primary antibody.

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