FGF acidic/FGF1 Antibody (OTI4D2)

Novus Biologicals | Catalog # NBP1-47748

Novus Biologicals
Discontinued Product
NBP1-47748 has been discontinued. View all FGF acidic/FGF1 products.

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4D2
View all FGF acidic/FGF1 Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 16-155 of human AFGF (NP_000791) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens fibroblast growth factor 1 (acidic) (FGF1), transcript variant 1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

15.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FGF acidic/FGF1 Antibody (OTI4D2)

Western Blot: FGF acidic/FGF1 Antibody (OTI4D2) [NBP1-47748]

Western Blot: FGF acidic/FGF1 Antibody (OTI4D2) [NBP1-47748]

Western Blot: FGF acidic/FGF1 Antibody (4D2) [NBP1-47748] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FGF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FGF1.
Immunocytochemistry/ Immunofluorescence: FGF acidic/FGF1 Antibody (OTI4D2) [NBP1-47748]

Immunocytochemistry/ Immunofluorescence: FGF acidic/FGF1 Antibody (OTI4D2) [NBP1-47748]

Immunocytochemistry/Immunofluorescence: FGF acidic/FGF1 Antibody (4D2) [NBP1-47748] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY FGF1.
Western Blot: FGF acidic/FGF1 Antibody (OTI4D2) [NBP1-47748]

Western Blot: FGF acidic/FGF1 Antibody (OTI4D2) [NBP1-47748]

Western Blot: FGF acidic/FGF1 Antibody (4D2) [NBP1-47748] - FGF1 antibody ( 4D2 ) at 1:50000 dilution + recombinant Human FGF1.

Applications for FGF acidic/FGF1 Antibody (OTI4D2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:5000-50000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FGF acidic/FGF1

FGF1 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described

Long Name

Fibroblast Growth Factor acidic

Alternate Names

alpha-ECGF, beta-ECGF, FGF acidic, FGF-1, HBGF-1

Entrez Gene IDs

2246 (Human)

Gene Symbol

FGF1

Additional FGF acidic/FGF1 Products

Product Documents for FGF acidic/FGF1 Antibody (OTI4D2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for FGF acidic/FGF1 Antibody (OTI4D2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs for FGF acidic/FGF1 Antibody (OTI4D2)

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  • Q: What is the heparin binding activity of FGF and VEGF?

    A: These proteins are not assayed for their ability to bind Heparin. More information about the FGF family of growth factors is available in this review article: Basilico, C. (1992) Adv. Can. Res. 59:115.

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