FGF acidic/FGF1 Antibody (OTI4D2) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-70717

Novus Biologicals
Discontinued Product
NBP2-70717 has been discontinued. View all FGF acidic/FGF1 products.

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4D2

Format

Azide and BSA Free
View all FGF acidic/FGF1 Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 16-155 of human AFGF (NP_000791) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

15.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FGF acidic/FGF1 Antibody (OTI4D2) - Azide and BSA Free

Western Blot: FGF acidic/FGF1 Antibody (OTI4D2)Azide and BSA Free [NBP2-70717]

Western Blot: FGF acidic/FGF1 Antibody (OTI4D2)Azide and BSA Free [NBP2-70717]

Western Blot: FGF acidic/FGF1 Antibody (OTI4D2) - Azide and BSA Free [NBP2-70717] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FGF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FGF1.
Immunocytochemistry/ Immunofluorescence: FGF acidic/FGF1 Antibody (OTI4D2) - Azide and BSA Free [NBP2-70717]

Immunocytochemistry/ Immunofluorescence: FGF acidic/FGF1 Antibody (OTI4D2) - Azide and BSA Free [NBP2-70717]

Immunocytochemistry/Immunofluorescence: FGF acidic/FGF1 Antibody (OTI4D2) - Azide and BSA Free [NBP2-70717] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY FGF1.
Western Blot: FGF acidic/FGF1 Antibody (OTI4D2)Azide and BSA Free [NBP2-70717]

Western Blot: FGF acidic/FGF1 Antibody (OTI4D2)Azide and BSA Free [NBP2-70717]

Western Blot: FGF acidic/FGF1 Antibody (OTI4D2) - Azide and BSA Free [NBP2-70717] - FGF1 antibody ( 4D2 ) at 1:50000 dilution + recombinant Human FGF1.

Applications for FGF acidic/FGF1 Antibody (OTI4D2) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:5000-50000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGF acidic/FGF1

FGF acidic/FGF1, also known as ECGF and HBGF-1, is a secreted mitogen that stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. It plays a number of roles in development, regeneration, and angiogenesis. FGF1 is released extracellularly as a disulfide-linked homodimer and is stored in complex with extracellular heparan sulfate. The association of FGF1 with heparan sulfate is a prerequisite for its subsequent interaction with FGF receptors. Internalized FGF1 can translocate to the cytosol and to the nucleus. Intracellular FGF1 functions as a survival factor by inhibiting p53 activity and proapoptotic signaling.

Long Name

Fibroblast Growth Factor acidic

Alternate Names

alpha-ECGF, beta-ECGF, FGF acidic, FGF-1, HBGF-1

Gene Symbol

FGF1

Additional FGF acidic/FGF1 Products

Product Documents for FGF acidic/FGF1 Antibody (OTI4D2) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for FGF acidic/FGF1 Antibody (OTI4D2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs for FGF acidic/FGF1 Antibody (OTI4D2) - Azide and BSA Free

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  • Q: What is the heparin binding activity of FGF and VEGF?

    A: These proteins are not assayed for their ability to bind Heparin. More information about the FGF family of growth factors is available in this review article: Basilico, C. (1992) Adv. Can. Res. 59:115.

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