FGF activity is mediated by a family of type I transmembrane tyrosine kinases, which undergo dimerization and autophosphorylation after ligand binding. Five distinct genes encode closely related FGF receptors, FGFR1 through 5. FGFRs contain three Ig-like domains and a stretch of acidic residues between the first and second Ig-like domains. FGFR1, 2, 3, and -4 have a cytoplasmic split tyrosine-kinase domain, but FGFR5 does not. Multiple forms of FGFR1, 2, and 3 are generated by alternative splicing.
FGFR1 Antibody (133111) [Allophycocyanin]
Novus Biologicals | Catalog # FAB765A
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Neutralization
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 133111
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Product Specifications
Immunogen
A mixture of purified isoforms of recombinant human FGF R1 extracellular domains
Specificity
Detects IIIb isoforms of human FGF R1. In direct ELISAs and Western blots, this antibody detected IIIb but not IIIc isoforms of human FGF R1, human FGF R2, and mouse FGF R2. In direct ELISA, approximately 20% cross-reactivity was observed with recombinant human (rh) FGF R3 (IIIb). No cross-reactivity was observed with rhFGF R4 or with IIIIc isoforms of rhFGF R1, rhFGF R2, rmFGF R2, or rhFGF R3.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for FGFR1 Antibody (133111) [Allophycocyanin]
Application
Recommended Usage
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Neutralization
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: FGFR1
Long Name
Fibroblast Growth Factor Receptor 1
Alternate Names
CD331, FGF R1, Flt-2
Gene Symbol
FGFR1
Additional FGFR1 Products
Product Documents for FGFR1 Antibody (133111) [Allophycocyanin]
Product Specific Notices for FGFR1 Antibody (133111) [Allophycocyanin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for FGFR1 Antibody (133111) [Allophycocyanin]
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Q: I am working in Multiple sclerosis group and mainly working with mice model and cell culture study. I need FGFR1 antibody against Mice for FACS analysis. If it is available can I get the sample to check with my cells? And any Oligodendrocyte markers against mice for FACS too.
A: We do have an antibody that has been tested in FLOW, NB600-1287, however it has not been tested in mouse. If you would like to test this antibody in mouse samples we have an Innovators Reward Program where we reward you for trying our antibody in species and applications that have not been previously tested. -
Q: I would like to inquire whether you have samples of the following antibodies: NBP1-61338, NB600-1287, NBP1-19481, NBP1-19864; The datasheets for these antibodies show different molecular weights of the detected proteins so its no altogether clear to me which one detects the real protein. To sort this out, we would be using FGFR1-deficient cells and would very much appreciate if you could supply us with small aliquots of these antibodies.
A: FGFR1 has multiple isoforms and is subject to multiple PTM's. The molecular weight can vary greatly depending on experimental conditions and samples used. We fully guarantee all of our products for the listed applications and species. If you cannot get a product to work in an application or species stated on our datasheet, our technical service team will troubleshoot with you to get it to work. If a product still does not work after troubleshooting, you can receive a free of charge replacement product or a full refund. Due to our 100% guarantee, we do not offer free of charge samples of our products. If a smaller sample size is available, it will be listed on our product page.
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Associated Pathways
Neural Stem Cell Differentiation Pathways & Lineage-specific Markers
