Human FGFR1 (IIIb) Antibody

Catalog # Availability Size / Price Qty
MAB765-SP
MAB765-500
MAB765-100
FGF R1 alpha  Inhibition of FGF acidic-dependent Cell Proliferation and Neutralization by Human FGF R1 Antibody.
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Product Details
Citations (2)
FAQs
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Human FGFR1 (IIIb) Antibody Summary

Species Reactivity
Human
Specificity
Detects IIIb isoforms of human FGF R1. In direct ELISAs and Western blots, this antibody detected IIIb but not IIIc isoforms of human FGF R1, human FGF R2, and mouse FGF R2. In direct ELISA, approximately 20% cross-reactivity was observed with recombinant human (rh) FGF R3 (IIIb). No cross-reactivity was observed with rhFGF R4 or with IIIIc isoforms of rhFGF R1, rhFGF R2, rmFGF R2, or rhFGF R3.
Source
Monoclonal Mouse IgG1 Clone # 133111
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
A mixture of purified isoforms of recombinant human FGF R1 extracellular domains
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human FGF R1 alpha (IIIb) Fc Chimera (Catalog # 655-FR)
Neutralization
Measured by its ability to neutralize FGF R1 alpha -mediated inhibition of proliferation in the NR6R‑3T3 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 0.075-0.3 µg/mL in the presence of 6 ng/mL Recombinant Human FGF R1 alpha (IIIb) Fc Chimera, 0.3 ng/mL Recombinant Human FGF acidic, and 10 µg/mL heparin.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization FGF R1 alpha  Inhibition of FGF acidic-dependent Cell Proliferation and Neutralization by Human FGF R1 Antibody. View Larger

FGF R1 alpha Inhibition of FGF acidic-dependent Cell Proliferation and Neutralization by Human FGF R1 Antibody. Recombinant Human FGF R1a (IIIb) Fc Chimera (Catalog # 655-FR) inhibits Recombinant Human FGF acidic (Catalog # 232-FA) induced proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human FGF acidic (0.3 ng/mL) activity elicited by Recombinant Human FGF R1a (IIIb) Fc Chimera (6 ng/mL) is neutralized (green line) by increasing concentrations of Human FGF R1 (IIIb) Monoclonal Antibody (Catalog # MAB765). The ND50 is typically 0.075-0.3 µg/mL in the presence of heparin (10 µg/mL).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGFR1

FGF R1 occurs as multiple isoforms. The extracellular domain consists of three ( alpha isoforms) or two ( beta isoforms) immunoglobulin-like domains. In addition, the membrane-proximal Ig-like domain can be encoded by alternative exons yielding (IIIb) or (IIIc) isoforms.

Long Name
Fibroblast Growth Factor Receptor 1
Entrez Gene IDs
2260 (Human); 14182 (Mouse)
Alternate Names
basic fibroblast growth factor receptor 1; BFGFR; bFGF-R-1; CD331 antigen; CD331; CEK; EC 2.7.10; EC 2.7.10.1; FGF R1; FGFBR; FGFR1; FGFR-1; fibroblast growth factor receptor 1; FLGH3; FLJ99988; Flt-2; FLT2H4; Fms-like tyrosine kinase 2; fms-related tyrosine kinase 2; H2; H5; HBGFR; heparin-binding growth factor receptor; hydroxyaryl-protein kinase; KAL 2; KAL2; N-SAM; OGD; Proto-oncogene c-Fgr; soluble FGFR1 variant 1; soluble FGFR1 variant 2

Product Datasheets

Citations for Human FGFR1 (IIIb) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Fibroblast growth factor-2 maintains a niche-dependent population of self-renewing highly potent non-adherent mesenchymal progenitors through FGFR2c.
    Authors: Di Maggio N, Mehrkens A, Papadimitropoulos A, Schaeren S, Heberer M, Banfi A, Martin I
    Stem Cells, 2012;30(7):1455-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Regulation of platelet-derived growth factor receptor function by integrin-associated cell surface transglutaminase.
    Authors: Zemskov EA, Loukinova E, Mikhailenko I, Coleman RA, Strickland DK, Belkin AM
    J. Biol. Chem., 2009;284(24):16693-703.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

  1. Why is heparin necessary in the neutralization assay using Human FGFR1 (IIIb) Antibody, Catalog # MAB765?

    • The heparin is a necessary cofactor for the binding of FGF to the receptor which induces the proliferation that this antibody neutralizes.

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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