FGFR1 Antibody (MM0277-6F10) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-12308

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Block/Neutralize

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # MM0277-6F10

Format

Azide and BSA Free
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Product Specifications

Immunogen

Human recombinant FGFR1 (extracellular domains of mixed isoforms)

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:25024372).

Specificity

Detect human FGF-R1 IIIb isoform only

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for FGFR1 Antibody (MM0277-6F10) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Reconstitution

Reconstitute with sterilized PBS to a final concentration of 0.5 mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGFR1

FGF activity is mediated by a family of type I transmembrane tyrosine kinases, which undergo dimerization and autophosphorylation after ligand binding. Five distinct genes encode closely related FGF receptors, FGFR1 through 5. FGFRs contain three Ig-like domains and a stretch of acidic residues between the first and second Ig-like domains. FGFR1, 2, 3, and -4 have a cytoplasmic split tyrosine-kinase domain, but FGFR5 does not. Multiple forms of FGFR1, 2, and 3 are generated by alternative splicing.

Long Name

Fibroblast Growth Factor Receptor 1

Alternate Names

CD331, FGF R1, Flt-2

Gene Symbol

FGFR1

UniProt

Additional FGFR1 Products

Product Documents for FGFR1 Antibody (MM0277-6F10) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for FGFR1 Antibody (MM0277-6F10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for FGFR1 Antibody (MM0277-6F10) - Azide and BSA Free

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FAQs for FGFR1 Antibody (MM0277-6F10) - Azide and BSA Free

Showing  1 - 3 of 3 FAQs Showing All
  • Q: I am working in Multiple sclerosis group and mainly working with mice model and cell culture study. I need FGFR1 antibody against Mice for FACS analysis. If it is available can I get the sample to check with my cells? And any Oligodendrocyte markers against mice for FACS too.

    A:

    We do have an antibody that has been tested in FLOW, NB600-1287, however it has not been tested in mouse. If you would like to test this antibody in mouse samples we have an Innovators Reward Program where we reward you for trying our antibody in species and applications that have not been previously tested.

  • Q: I would like to inquire whether you have samples of the following antibodies: NBP1-61338, NB600-1287, NBP1-19481, NBP1-19864; The datasheets for these antibodies show different molecular weights of the detected proteins so its no altogether clear to me which one detects the real protein. To sort this out, we would be using FGFR1-deficient cells and would very much appreciate if you could supply us with small aliquots of these antibodies.

    A: FGFR1 has multiple isoforms and is subject to multiple PTM's. The molecular weight can vary greatly depending on experimental conditions and samples used. We fully guarantee all of our products for the listed applications and species. If you cannot get a product to work in an application or species stated on our datasheet, our technical service team will troubleshoot with you to get it to work. If a product still does not work after troubleshooting, you can receive a free of charge replacement product or a full refund. Due to our 100% guarantee, we do not offer free of charge samples of our products. If a smaller sample size is available, it will be listed on our product page.

  • Q: What is the recommended concentration for Blocking/Neutralizing?

    A: When performing blocking/neutralizing experiments with another anti-human FGFR1 antibody, please use the molar ration of 50 - 200 molar in excess; for example: If NBP2-12308 will be used to block/neutralize antibody A in WB and it need 1 ug/ml of A for the WB experiments, then please do this calculations: 1), Antibody A's molar concentration: 1 ug divides by 160 g/mole in 1 ml [we regularly use 160 kDa as the MW for one IgG molecule], and say it equals to the value of N. 2), Then the amount that is required for using NBP2-12308 in blocking/neutralizing will be 50 - 200 times N. 3) And use the above result in #2 (the number) for calculate how many ug of NBP2-12308 will be needed (again use 160 kDa per IgG to calculate) to add to the reaction solution for blocking/neutralizing.

  • Q: I am working in Multiple sclerosis group and mainly working with mice model and cell culture study. I need FGFR1 antibody against Mice for FACS analysis. If it is available can I get the sample to check with my cells? And any Oligodendrocyte markers against mice for FACS too.

    A:

    We do have an antibody that has been tested in FLOW, NB600-1287, however it has not been tested in mouse. If you would like to test this antibody in mouse samples we have an Innovators Reward Program where we reward you for trying our antibody in species and applications that have not been previously tested.

  • Q: I would like to inquire whether you have samples of the following antibodies: NBP1-61338, NB600-1287, NBP1-19481, NBP1-19864; The datasheets for these antibodies show different molecular weights of the detected proteins so its no altogether clear to me which one detects the real protein. To sort this out, we would be using FGFR1-deficient cells and would very much appreciate if you could supply us with small aliquots of these antibodies.

    A: FGFR1 has multiple isoforms and is subject to multiple PTM's. The molecular weight can vary greatly depending on experimental conditions and samples used. We fully guarantee all of our products for the listed applications and species. If you cannot get a product to work in an application or species stated on our datasheet, our technical service team will troubleshoot with you to get it to work. If a product still does not work after troubleshooting, you can receive a free of charge replacement product or a full refund. Due to our 100% guarantee, we do not offer free of charge samples of our products. If a smaller sample size is available, it will be listed on our product page.

  • Q: What is the recommended concentration for Blocking/Neutralizing?

    A: When performing blocking/neutralizing experiments with another anti-human FGFR1 antibody, please use the molar ration of 50 - 200 molar in excess; for example: If NBP2-12308 will be used to block/neutralize antibody A in WB and it need 1 ug/ml of A for the WB experiments, then please do this calculations: 1), Antibody A's molar concentration: 1 ug divides by 160 g/mole in 1 ml [we regularly use 160 kDa as the MW for one IgG molecule], and say it equals to the value of N. 2), Then the amount that is required for using NBP2-12308 in blocking/neutralizing will be 50 - 200 times N. 3) And use the above result in #2 (the number) for calculate how many ug of NBP2-12308 will be needed (again use 160 kDa per IgG to calculate) to add to the reaction solution for blocking/neutralizing.

  • Q: I am working in Multiple sclerosis group and mainly working with mice model and cell culture study. I need FGFR1 antibody against Mice for FACS analysis. If it is available can I get the sample to check with my cells? And any Oligodendrocyte markers against mice for FACS too.

    A:

    We do have an antibody that has been tested in FLOW, NB600-1287, however it has not been tested in mouse. If you would like to test this antibody in mouse samples we have an Innovators Reward Program where we reward you for trying our antibody in species and applications that have not been previously tested.

  • Q: I would like to inquire whether you have samples of the following antibodies: NBP1-61338, NB600-1287, NBP1-19481, NBP1-19864; The datasheets for these antibodies show different molecular weights of the detected proteins so its no altogether clear to me which one detects the real protein. To sort this out, we would be using FGFR1-deficient cells and would very much appreciate if you could supply us with small aliquots of these antibodies.

    A: FGFR1 has multiple isoforms and is subject to multiple PTM's. The molecular weight can vary greatly depending on experimental conditions and samples used. We fully guarantee all of our products for the listed applications and species. If you cannot get a product to work in an application or species stated on our datasheet, our technical service team will troubleshoot with you to get it to work. If a product still does not work after troubleshooting, you can receive a free of charge replacement product or a full refund. Due to our 100% guarantee, we do not offer free of charge samples of our products. If a smaller sample size is available, it will be listed on our product page.

  • Q: What is the recommended concentration for Blocking/Neutralizing?

    A: When performing blocking/neutralizing experiments with another anti-human FGFR1 antibody, please use the molar ration of 50 - 200 molar in excess; for example: If NBP2-12308 will be used to block/neutralize antibody A in WB and it need 1 ug/ml of A for the WB experiments, then please do this calculations: 1), Antibody A's molar concentration: 1 ug divides by 160 g/mole in 1 ml [we regularly use 160 kDa as the MW for one IgG molecule], and say it equals to the value of N. 2), Then the amount that is required for using NBP2-12308 in blocking/neutralizing will be 50 - 200 times N. 3) And use the above result in #2 (the number) for calculate how many ug of NBP2-12308 will be needed (again use 160 kDa per IgG to calculate) to add to the reaction solution for blocking/neutralizing.

Showing  1 - 3 of 3 FAQs Showing All
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