Fibrinogen gamma chain Antibody (OTI2D2) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-70732

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2D2

Format

Azide and BSA Free
View all Fibrinogen gamma chain Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human FGG(NP_000500) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

46.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Fibrinogen gamma chain Antibody (OTI2D2) - Azide and BSA Free

Western Blot: Fibrinogen gamma chain Antibody (OTI2D2)Azide and BSA Free [NBP2-70732]

Western Blot: Fibrinogen gamma chain Antibody (OTI2D2)Azide and BSA Free [NBP2-70732]

Western Blot: Fibrinogen gamma chain Antibody (OTI2D2) - Azide and BSA Free [NBP2-70732] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Fibrinogen gamma chain.
Immunohistochemistry: Fibrinogen gamma chain Antibody (OTI2D2) - Azide and BSA Free [NBP2-70732]

Immunohistochemistry: Fibrinogen gamma chain Antibody (OTI2D2) - Azide and BSA Free [NBP2-70732]

Immunohistochemistry: Fibrinogen gamma chain Antibody (OTI2D2) - Azide and BSA Free [NBP2-70732] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Western Blot: Fibrinogen gamma chain Antibody (OTI2D2)Azide and BSA Free [NBP2-70732]

Western Blot: Fibrinogen gamma chain Antibody (OTI2D2)Azide and BSA Free [NBP2-70732]

Western Blot: Fibrinogen gamma chain Antibody (OTI2D2) - Azide and BSA Free [NBP2-70732] - Analysis of extracts (35ug) from 9 different cell lines by using FGG monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemistry: Fibrinogen gamma chain Antibody (OTI2D2) - Azide and BSA Free [NBP2-70732]

Immunohistochemistry: Fibrinogen gamma chain Antibody (OTI2D2) - Azide and BSA Free [NBP2-70732]

Immunohistochemistry: Fibrinogen gamma chain Antibody (OTI2D2) - Azide and BSA Free [NBP2-70732] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Fibrinogen gamma chain Antibody (OTI2D2) - Azide and BSA Free [NBP2-70732]

Immunohistochemistry: Fibrinogen gamma chain Antibody (OTI2D2) - Azide and BSA Free [NBP2-70732]

Immunohistochemistry: Fibrinogen gamma chain Antibody (OTI2D2) - Azide and BSA Free [NBP2-70732] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Fibrinogen gamma chain Antibody (OTI2D2) - Azide and BSA Free [NBP2-70732]

Immunohistochemistry: Fibrinogen gamma chain Antibody (OTI2D2) - Azide and BSA Free [NBP2-70732]

Immunohistochemistry: Fibrinogen gamma chain Antibody (OTI2D2) - Azide and BSA Free [NBP2-70732] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Fibrinogen gamma chain Antibody (OTI2D2) - Azide and BSA Free [NBP2-70732]

Immunohistochemistry: Fibrinogen gamma chain Antibody (OTI2D2) - Azide and BSA Free [NBP2-70732]

Immunohistochemistry: Fibrinogen gamma chain Antibody (OTI2D2) - Azide and BSA Free [NBP2-70732] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Applications for Fibrinogen gamma chain Antibody (OTI2D2) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:400-4000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fibrinogen gamma chain

The protein encoded by the Fibrinogen gamma chain gene is the gamma component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia and thrombophilia. Alternative splicing results in two transcript variants encoding different isoforms.

Alternate Names

fibrinogen gamma chain, fibrinogen, gamma polypeptide

Gene Symbol

FGG

Additional Fibrinogen gamma chain Products

Product Documents for Fibrinogen gamma chain Antibody (OTI2D2) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Fibrinogen gamma chain Antibody (OTI2D2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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