Fibroblast Activation Protein alpha/FAP Antibody - BSA Free

Novus Biologicals | Catalog # NB110-85534

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, ELISA, Flow Cytometry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Fibroblast Activation Protein alpha/FAP Primary Antibodies »

Product Specifications

Immunogen

This affinity purified Fibroblast Activation Protein alpha/FAP Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse Fibroblast Activation Protein alpha/Fibroblast Activation Protein alpha/FAP protein. (Uniprot: P97321)

Reactivity Notes

A BLAST analysis was used to suggest partial cross-reactivity with Fibroblast Activation Protein alpha/FAP from human and rat sources based on ~88% homology with the immunizing sequence. Reactivity with Fibroblast Activation Protein alpha/FAP from other sources has not been determined. Human reactivity reported from a verified customer review.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This product was affinity purified from monospecific antiserum by immunoaffinity chromatography

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Fibroblast Activation Protein alpha/FAP Antibody - BSA Free

Western Blot: Fibroblast Activation Protein alpha/FAP Antibody [NB110-85534]

Western Blot: Fibroblast Activation Protein alpha/FAP Antibody [NB110-85534]

Western Blot: Fibroblast Activation Protein alpha/FAP Antibody [NB110-85534] - Western blot using affinity purified anti-Fibroblast Activation protein alpha/Fibroblast Activation Protein alpha/FAP antibody shows detection of Fibroblast Activation Protein alpha/FAP protein in whole cell lysates from Fibroblast Activation Protein alpha/FAP expressing HEK cells (lane 4) but not control HEK cells (lane 3). Specific band staining is blocked when the primary antibody is pre-incubated with immunizing peptide (lanes 1 and 2 respectively). The band at ~90 kDa, indicated by the arrowhead, corresponds to the expected molecular weight of transfected Fibroblast Activation Protein alpha/FAP. Primary antibody was used at 1:1,000.
ELISA: Fibroblast Activation Protein alpha/FAP Antibody [NB110-85534]

ELISA: Fibroblast Activation Protein alpha/FAP Antibody [NB110-85534]

ELISA: Fibroblast Activation Protein alpha/FAP Antibody [NB110-85534] - ELISA results of purified Fibroblast Activation protein alpha/FAP antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate.
Fibroblast Activation Protein alpha/FAP Antibody

Flow Cytometry: Fibroblast Activation Protein alpha/FAP Antibody [NB110-85534]

Flow Cytometry: Fibroblast Activation Protein alpha/FAP Antibody [NB110-85534] - Specific co-culture system created to study human macrophage cancer cells' conduction of MMT process, and Fibroblast Activation Protein alpha/FAP Antibody [Alexa Fluor® 488] (Catalog # NB110-85534AF488) was used as a myo-CAFs marker. Image from a verified customer review.
Fibroblast Activation Protein alpha/FAP Antibody - BSA Free

Western Blot: Fibroblast Activation Protein alpha/FAP Antibody - BSA Free [NB110-85534] -

Identification of rAd‐infected DCs in vitro. (A–C) rAd‐FAP, rAd‐hlivin alpha, and rAd‐FAP/hlivin alpha were constructed using mouse FAP cDNA and human livin alpha cDNA, and then transduced into mouse bone marrow‐derived DCs, respectively. For evaluation of transduction efficacy, western blot was performed to measure protein expressions of FAP and livin alpha in cells, with GAPDH functioned as a loading control. ***p <.001, versus 1#; +++p <.001, versus 2#; ^^^p <.001, versus 3#. DCs, dendritic cells; FAP, fibroblast activation protein‐ alpha ; rAd‐FAP, recombinant adenoviral vector encoding mouse FAP cDNA; rAd‐hlivin alpha, recombinant adenoviral vector encoding human livin alpha cDNA. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37773704), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Fibroblast Activation Protein alpha/FAP Antibody - BSA Free

Application
Recommended Usage

ELISA

1:10000-1:20000

Flow Cytometry

Validated for Flow Cytometry from a verified customer review.

Western Blot

1:500-1:2000
Application Notes
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 88 kDa in size corresponding to FAP by western blotting in the appropriate cell lysate or extract.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: Fibroblast Activation Protein alpha/FAP

Fibroblast Activation Protein (FAP) is expressed in the stroma of sites that are undergoing wound healing. In addition, it has recently been reported that FAP is expressed in the stroma of sites of metastatic disease. Inhibition of FAP may lead to a dramatic decrease in the number of metastatic osteosarcoma lung nodules. FAP exists as an inactive monomer and when activated forms homodimers or heterodimers with DPP4. Multiple isoforms of FAP are reported as alternative splicing products from a common gene.

Alternate Names

FAP, Seprase

Entrez Gene IDs

2191 (Human); 14089 (Mouse); 192203 (Rat)

Gene Symbol

FAP

UniProt

P97321, Q12884

Additional Fibroblast Activation Protein alpha/FAP Products

Product Documents for Fibroblast Activation Protein alpha/FAP Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Fibroblast Activation Protein alpha/FAP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Fibroblast Activation Protein alpha/FAP Antibody - BSA Free

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Protocols

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