Fibroblast Activation Protein alpha/FAP Antibody [Alexa Fluor® 488]

Novus Biologicals | Catalog # NB110-85534AF488

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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Polyclonal Rabbit IgG
View all Fibroblast Activation Protein alpha/FAP Primary Antibodies »

Product Specifications

Immunogen

This affinity purified Fibroblast Activation Protein alpha/FAP Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse Fibroblast Activation Protein alpha/Fibroblast Activation Protein alpha/FAP protein. (Uniprot: P97321)

Reactivity Notes

A BLAST analysis was used to suggest partial cross-reactivity with Fibroblast Activation Protein alpha/FAP from human and rat sources based on ~88% homology with the immunizing sequence. Reactivity with Fibroblast Activation Protein alpha/FAP from other sources has not been determined. Human reactivity reported from a verified customer review.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Fibroblast Activation Protein alpha/FAP Antibody [Alexa Fluor® 488]

Fibroblast Activation Protein alpha/FAP Antibody [Alexa Fluor® 488]

Flow Cytometry: Fibroblast Activation Protein alpha/FAP Antibody [Alexa Fluor® 488] [NB110-85534AF488]

Flow Cytometry: Fibroblast Activation Protein alpha/FAP Antibody [Alexa Fluor® 488] [NB110-85534AF488] - Specific co-culture system created to study human macrophage cancer cells' conduction of MMT process, and Fibroblast Activation Protein alpha/FAP Antibody [Alexa Fluor® 488] (Catalog # NB110-85534AF488) was used as a myo-CAFs marker. Image from a verified customer review.

Applications for Fibroblast Activation Protein alpha/FAP Antibody [Alexa Fluor® 488]

Application
Recommended Usage

Flow Cytometry

Validated for Flow Cytometry from a verified customer review.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NB110-85534AF488 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Fibroblast Activation Protein alpha/FAP

Fibroblast Activation Protein (FAP) is expressed in the stroma of sites that are undergoing wound healing. In addition, it has recently been reported that FAP is expressed in the stroma of sites of metastatic disease. Inhibition of FAP may lead to a dramatic decrease in the number of metastatic osteosarcoma lung nodules. FAP exists as an inactive monomer and when activated forms homodimers or heterodimers with DPP4. Multiple isoforms of FAP are reported as alternative splicing products from a common gene.

Alternate Names

FAP, Seprase

Gene Symbol

FAP

Additional Fibroblast Activation Protein alpha/FAP Products

Product Documents for Fibroblast Activation Protein alpha/FAP Antibody [Alexa Fluor® 488]

Certificate of Analysis

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Product Specific Notices for Fibroblast Activation Protein alpha/FAP Antibody [Alexa Fluor® 488]



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews (1)

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Customer Images

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  • Fibroblast Activation Protein alpha/FAP Antibody [Alexa Fluor® 488]
    Name: Shinford Sun
    Application: Flow Cytometry
    Sample Tested: human macrophage
    Species: Human
    Verified Customer | Posted 09/24/2024
    We created a specific co-culture system to study the cancer cells' conduction of MMT process, and FAP was used as a myo-CAFs marker.
    Fibroblast Activation Protein alpha/FAP Antibody [Alexa Fluor® 488] NB110-85534AF488
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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