FIP1/RCP Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-37698

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all FIP1/RCP Primary Antibodies »

Product Specifications

Immunogen

This antibody maps to a region between residue 675 to 725 of human RAB11 Coupling Protein using the numbering given in entry NP_001002814.2 (GeneID 80223).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FIP1/RCP Antibody - BSA Free

Western Blot: FIP1/RCP Antibody [NBP2-37698]

Western Blot: FIP1/RCP Antibody [NBP2-37698]

Western Blot: FIP1/RCP Antibody [NBP2-37698] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Affinity purified rabbit anti-RCP antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: FIP1/RCP Antibody [NBP2-37698]

Immunoprecipitation: FIP1/RCP Antibody [NBP2-37698]

Immunoprecipitation: FIP1/RCP Antibody [NBP2-37698] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Affinity purified rabbit anti-RCP antibody used for IP at 6 ug per reaction. RCP was also immunoprecipitated by rabbit anti-RCP antibody. For blotting immunoprecipitated RCP, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for FIP1/RCP Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: FIP1/RCP

Proteins of the large Rab GTPase family have regulatory roles in the formation, targeting, and fusion of intracellular transport vesicles. RAB11FIP1 is one of many proteins that interact with and regulate Rab GTPases (Hales et al., 2001)

Alternate Names

DKFZp686E2214, EC 3.2.1.28, EC 6.1.1.7, FLJ22524, FLJ22622, MGC78448, NOEL1A, RAB11 coupling protein, RAB11 family interacting protein 1 (class I), rab11 family-interacting protein 1, rab11-FIP1, Rab-coupling protein, Rab-interacting recycling protein, RCPRab effector protein

Gene Symbol

RAB11FIP1

UniProt

NP_001002814.2 (Human)

Additional FIP1/RCP Products

Product Documents for FIP1/RCP Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for FIP1/RCP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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