FITC is a small organic fluorochrome dye commonly used for a variety of labeling applications such as conjugation to antibodies or other proteins. Excitation of FITC results in emission of a visible yellow-green signal.
FITC Antibody (931845) [DyLight 405]
Novus Biologicals | Catalog # FAB8980E
Key Product Details
Species Reactivity
Multi-Species
Applications
Immunocytochemistry
Label
DyLight 405 (Excitation = 400 nm, Emission = 420 nm)
Antibody Source
Monoclonal Mouse IgG Clone # 931845
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Product Specifications
Immunogen
Fluorescein Isothiocyanate (FITC)
Specificity
Detects Fluorescein Isothiocyanate (FITC) and Carboxyfluorescein (CFS) in direct ELISAs. In direct ELISAs, no cross-reactivity with Alexa Fluor® 488, Alexa Fluor® 594, Alexa Fluor® 647, Alexa Fluor® 700, Alexa Fluor® 750, NorthernLights™ NL493, NorthernLights™ 557, and NorthernLights™ 637 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG
Applications for FITC Antibody (931845) [DyLight 405]
Application
Recommended Usage
Immunocytochemistry
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: FITC
Long Name
Fluorescein Isothiocyanate
Alternate Names
Fluorescein Isothiocyanate
Additional FITC Products
Product Documents for FITC Antibody (931845) [DyLight 405]
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for FITC Antibody (931845) [DyLight 405]
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for FITC Antibody (931845) [DyLight 405]
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Q: What is the molecular weight of Fluorescein Isothiocyanate (FITC)?
A: The molecular weight of FITC is 389.38 Daltons. However, R&D Systems uses 5- (and 6-) Carboxyfluorescein (CFS) for antibody conjugation, which has a molecular weight of 473 Daltons.
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