Key Product Details

Species Reactivity

Multi-Species

Applications

Immunocytochemistry

Label

CoraFluor 1

Antibody Source

Monoclonal Mouse IgG Clone # 931845
View all FITC Primary Antibodies »

Product Specifications

Immunogen

Fluorescein Isothiocyanate (FITC)

Specificity

Detects Fluorescein Isothiocyanate (FITC) and Carboxyfluorescein (CFS) in direct ELISAs. In direct ELISAs, no cross-reactivity with Alexa Fluor® 488, Alexa Fluor® 594, Alexa Fluor® 647, Alexa Fluor® 700, Alexa Fluor® 750, NorthernLights™ NL493, NorthernLights™ 557, and NorthernLights™ 637 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

CoraFluor(TM) 1, amine reactive

CoraFluor(TM) 1, thiol reactive

For more information, please see our CoraFluor(TM) TR-FRET technology flyer.

Scientific Data Images for FITC Antibody (931845) [CoraFluor™ 1]

FITC Antibody (931845) [CoraFluor™ 1]

Product Feature: CoraFluor Probes for TR-FRET

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

Applications for FITC Antibody (931845) [CoraFluor™ 1]

Application
Recommended Usage

Immunocytochemistry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: FITC

FITC is a small organic fluorochrome dye commonly used for a variety of labeling applications such as conjugation to antibodies or other proteins. Excitation of FITC results in emission of a visible yellow-green signal.

Long Name

Fluorescein Isothiocyanate

Alternate Names

Fluorescein Isothiocyanate

Additional FITC Products

Product Documents for FITC Antibody (931845) [CoraFluor™ 1]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for FITC Antibody (931845) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for FITC Antibody (931845) [CoraFluor™ 1]

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  • Q: What is the molecular weight of Fluorescein Isothiocyanate (FITC)?

    A: The molecular weight of FITC is 389.38 Daltons. However, R&D Systems uses 5- (and 6-) Carboxyfluorescein (CFS) for antibody conjugation, which has a molecular weight of 473 Daltons.

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