FKBP12 Antibody (OTI3B3) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-70741

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI3B3

Format

Azide and BSA Free
View all FKBP12 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human FKBP1A (NP_000792) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

11.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FKBP12 Antibody (OTI3B3) - Azide and BSA Free

Western Blot: FKBP12 Antibody (OTI3B3)Azide and BSA Free [NBP2-70741]

Western Blot: FKBP12 Antibody (OTI3B3)Azide and BSA Free [NBP2-70741]

Western Blot: FKBP12 Antibody (OTI3B3) - Azide and BSA Free [NBP2-70741] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FKBP12 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FKBP12.
Immunohistochemistry: FKBP12 Antibody (OTI3B3) - Azide and BSA Free [NBP2-70741]

Immunohistochemistry: FKBP12 Antibody (OTI3B3) - Azide and BSA Free [NBP2-70741]

Immunohistochemistry: FKBP12 Antibody (OTI3B3) - Azide and BSA Free [NBP2-70741] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-FKBP12 mouse monoclonal antibody.
Flow Cytometry: FKBP12 Antibody (OTI3B3) - Azide and BSA Free [NBP2-70741]

Flow Cytometry: FKBP12 Antibody (OTI3B3) - Azide and BSA Free [NBP2-70741]

Flow Cytometry: FKBP12 Antibody (OTI3B3) - Azide and BSA Free [NBP2-70741] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-FKBP12 antibody, and then analyzed by flow cytometry.
Immunohistochemistry: FKBP12 Antibody (OTI3B3) - Azide and BSA Free [NBP2-70741]

Immunohistochemistry: FKBP12 Antibody (OTI3B3) - Azide and BSA Free [NBP2-70741]

Immunohistochemistry: FKBP12 Antibody (OTI3B3) - Azide and BSA Free [NBP2-70741] - Staining of paraffin-embedded Human Kidney tissue using anti-FKBP12 mouse monoclonal antibody.

Applications for FKBP12 Antibody (OTI3B3) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Western Blot

1:200-1:1000

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FKBP12

Immunophilins are a family of soluble receptors capable of binding to one of two major immunosuppressant agents; cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Immunophilins function as cis-trans peptidyl-prolyl isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. Thus, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline-containing proteins.FKBP12:FK506 complexes inhibit calcineurin, a calcium/calmodulin-dependent serine/threonine phosphatase which blocks T-cell activation by preventing lymphokine gene transcription. FKBP12 also plays a role in intracellular calcium regulation by associating with three types of calcium release channel complexes, cardiac and skeletal ryanodine receptors and the inositol 1,4,5-trisphosphate receptor. In interactions with members of the TGF beta family type I receptors, FKBP12 has been shown to exert an inhibitory effect on the signaling pathway.

Long Name

12 kDa FK506 Binding Protein

Alternate Names

FKBP1a, PKC12, PPIASE

Gene Symbol

FKBP1A

Additional FKBP12 Products

Product Documents for FKBP12 Antibody (OTI3B3) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for FKBP12 Antibody (OTI3B3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Phosphatases and Regulators

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Protocols

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FAQs

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