FKBP12 Antibody

Novus Biologicals | Catalog # NB300-508

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all FKBP12 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to the N-terminal residues G(1) V Q V E T I S P G D G R(13) of human FKBP12.

Specificity

Detects FK506 binding protein 12 kDa (FKBP12).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FKBP12 Antibody

Western Blot: FKBP12 Antibody [NB300-508]

Western Blot: FKBP12 Antibody [NB300-508]

Western Blot: FKBP12 Antibody [NB300-508] - Analysis of purified FKBP12 after immunoprecipatation on a 15% gel.
Immunohistochemistry-Paraffin: FKBP12 Antibody [NB300-508]

Immunohistochemistry-Paraffin: FKBP12 Antibody [NB300-508]

Immunohistochemistry-Paraffin: FKBP12 Antibody [NB300-508] - Both normal and cancer biopsies of deparaffinized human Colon carcinoma tissue.
Immunohistochemistry-Paraffin: FKBP12 Antibody [NB300-508]

Immunohistochemistry-Paraffin: FKBP12 Antibody [NB300-508]

Immunohistochemistry-Paraffin: FKBP12 Antibody [NB300-508] - Both normal and cancer biopsies of deparaffinized human Heart tissue.
Immunohistochemistry-Paraffin: FKBP12 Antibody [NB300-508]

Immunohistochemistry-Paraffin: FKBP12 Antibody [NB300-508]

Immunohistochemistry-Paraffin: FKBP12 Antibody [NB300-508] - Both normal and cancer biopsies of deparaffinized human Tonsil tissue.

Applications for FKBP12 Antibody

Application
Recommended Usage

Immunohistochemistry

2 ug/ml

Immunohistochemistry-Paraffin

1:10 - 1:500

Immunoprecipitation

1:10 - 1:500

Western Blot

1 ug/ml
Application Notes
WB: Detects an approx. 12 kDa protein representing FKBP12 from rat heart homogenate.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FKBP12

Immunophilins are a family of soluble receptors capable of binding to one of two major immunosuppressant agents; cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Immunophilins function as cis-trans peptidyl-prolyl isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. Thus, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline-containing proteins.FKBP12:FK506 complexes inhibit calcineurin, a calcium/calmodulin-dependent serine/threonine phosphatase which blocks T-cell activation by preventing lymphokine gene transcription. FKBP12 also plays a role in intracellular calcium regulation by associating with three types of calcium release channel complexes, cardiac and skeletal ryanodine receptors and the inositol 1,4,5-trisphosphate receptor. In interactions with members of the TGF beta family type I receptors, FKBP12 has been shown to exert an inhibitory effect on the signaling pathway.

Long Name

12 kDa FK506 Binding Protein

Alternate Names

FKBP1a, PKC12, PPIASE

Entrez Gene IDs

2280 (Human); 14225 (Mouse); 25639 (Rat)

Gene Symbol

FKBP1A

UniProt

P62942

Additional FKBP12 Products

Product Documents for FKBP12 Antibody

Certificate of Analysis

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Product Specific Notices for FKBP12 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Phosphatases and Regulators

Citations for FKBP12 Antibody

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Protocols

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FAQs

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