FLI1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-57756

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Predicted:

Mouse (90%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all FLI1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: SYLRESSLLAYNTTSHTDQSSRLSVKEDPSYDSVRRGAWGNNMNSGLNKSPPLGGAQTISKNT

Reactivity Notes

Rat 87%

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FLI1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: FLI1 Antibody [NBP2-57756]

Immunocytochemistry/ Immunofluorescence: FLI1 Antibody [NBP2-57756]

Immunocytochemistry/Immunofluorescence: FLI1 Antibody [NBP2-57756] - Staining of human cell line U-2 OS shows localization to nucleus & nuclear bodies.
FLI1 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: FLI1 Antibody - BSA Free [NBP2-57756]

Chromatin Immunoprecipitation-exo-Seq: FLI1 Antibody - BSA Free [NBP2-57756]

ChIP-Exo-Seq composite graph for Anti-FLI1 (NBP2-57756) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for FLI1 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FLI1

Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. This family of genes currently includes Ets-1, Ets-2 Erg-1, Erg-2, Elk, E74, Fli-1, PU.1 and PEA3. Members of the Ets gene family exhibit varied pattern of tissue expression, and share a highly conserved carboxy terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence.

Long Name

Friend Leukemia Integration 1

Alternate Names

ERGB, SIC-1

Gene Symbol

FLI1

Additional FLI1 Products

Product Documents for FLI1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for FLI1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

ETS Family Transcription Factors

Citations for FLI1 Antibody - BSA Free

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