Human Flt-3 Ligand/FLT3L Recombinant Protein

Name: Human Flt-3 Ligand/FLT3L Recombinant Protein
Brand: Novus Biologicals
SKU: NBP2-36439
Price: 269 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-36439

Submit a review

Product Documents

100% Guarantee

Product Specifications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Source

Nso cells

Applications

Bioactivity, SDS-PAGE

View all Flt-3 Ligand/FLT3L Proteins and Enzymes »

Product Specifications

Description

A single glycosylated polypeptide chain protein corresponding to amino acids Gly27 - Arg188 of FLT3LG.

Source: Mouse myeloma cell line, NS0

Purity

SDS-PAGE

Endotoxin Level

Less than 1 EU/mg of the rMuFlt-3 Ligand as determined by LAL method.

Predicted Molecular Mass

Between 18-32 due to varying glycosylation kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

The ED50, as determined by a cell proliferation assay using Flt-3 transfected murine BaF3 cells, is less than 4 ng/ml.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation, and Storage

NBP2-36439

Preparation Method	Lyophilized from 0.2 mm filtered concentrated solution in 50% acetonitrile and 0.1% TFA.
Concentration	Lyoph
Reconstitution	Reconstitute with PBS to a final concentration of 0.05 mg/ml.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Flt-3 Ligand/FLT3L

Flt-3 Ligand promotes the differentiation of multiple hematopoietic cell lineages. Flt-3 Ligand is expressed as a noncovalently-linked dimer by T cells and bone marrow and thymic fibroblasts. Both transmembrane and soluble forms of Flt-3 Ligand signal through the tyrosine kinase receptor Flt-3/Flk-2. Flt-3 Ligand induces the expansion of monocytes and immature dendritic cells, B cell differentiation, NK cell development, and myeloid differentiation of hematopoietic stem cells. The mouse and human proteins show cross-species activity.

Long Name

fms-like Tyrosine Kinase 3 Ligand

Alternate Names

FLG3L, Flt3L, FLT3LG

Gene Symbol

FLT3LG

Additional Flt-3 Ligand/FLT3L Products

Product Documents for Human Flt-3 Ligand/FLT3L Recombinant Protein

Download Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human Flt-3 Ligand/FLT3L Recombinant Protein

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.